Cytoskeleton-associated protein 5 (CKAP5)

The protein contains 2032 amino acids for an estimated molecular weight of 225495 Da.

 

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments (PubMed:27156448). (updated: Jan. 31, 2018)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  6. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

This protein is annotated as membranous in Gene Ontology.


Interpro domains
Total structural coverage: 15%
Model score: 0
No model available.

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VariantDescription
dbSNP:rs11038988

The reference OMIM entry for this protein is 611142

Cytoskeleton-associated protein 5; ckap5
Colonic and hepatic tumor overexpressed gene; chtog
Mini spindles, drosophila, homolog of; msps
Kiaa0097

CLONING

By sequencing clones obtained from a size-fractionated immature myeloid cell line cDNA library, Nagase et al. (1995) cloned CKAP5, which they designated KIAA0097. The deduced protein contains 2,032 amino acids. Northern blot analysis detected highest expression in skeletal muscle and lower expression in all other tissues and cell lines examined, except peripheral blood leukocytes. Using TACC1 (605301) as bait in a yeast 2-hybrid screen of a mammary epithelial cDNA library, Lauffart et al. (2002) cloned CKAP5, which they called CHTOG. CHTOG contains a microtubule-binding domain in its N-terminal half and a KXGS motif for binding tubulin (see 191110) dimers in its C-terminal half.

GENE FUNCTION

Using RNA interference in HeLa cells, Gergely et al. (2003) found that TACC3 (605303) depletion did not alter spindle organization, but partly destabilized microtubules and redistributed CHTOG away from spindle microtubules. In CHTOG-depleted cells, relatively robust spindles formed, but they were highly disorganized. Gergely et al. (2003) concluded that CHTOG plays a major role in organizing spindle poles, whereas its role in stabilizing spindle microtubules is minor and, at least in part, mediated via interaction with TACC3. Brouhard et al. (2008) showed that the Xenopus CKAP5 ortholog, Xmap215, functioned as a processive microtubule polymerase. Recombinant Xmap215 bound free porcine brain tubulin in a 1:1 complex that interacted with the microtubule lattice and targeted the ends by a diffusion-facilitated mechanism. Xmap215 persisted at the plus end and catalyzed the addition of up to 25 tubulin dimers. Under some circumstances, Xmap215 catalyzed the reverse reaction, microtubule shrinkage.

MAPPING

Using human-rodent hybrid cell lines, Nagase et al. (1995) mapped the CKAP5 gene to chromosome 11. ... More on the omim web site

Subscribe to this protein entry history

Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 611142 was added.