Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (DHX15)
The protein contains 795 amino acids for an estimated molecular weight of 90933 Da.
Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. (updated: Jan. 31, 2018)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
Biological Process
MRNA processing
MRNA splicing, via spliceosome
Response to alkaloid
Response to toxic substance
RNA splicing
Cellular Component
Cytoplasm
Nuclear speck
Nucleolus
Nucleoplasm
Nucleus
Spliceosomal complex
U12-type spliceosomal complex
The reference OMIM entry for this protein is 603403
Deah box polypeptide 15; dhx15
Dead/h box 15; ddx15
Deah box protein 1; dbp1
Prp43, s. cerevisiae, homolog of
Rna helicase 2; hrh2
CLONING
By RT-PCR of HeLa cell mRNA with degenerate primers based on conserved regions of DEAH box proteins, Ono et al. (1994) isolated partial cDNAs encoding 5 members of the human DEAH box family, including HRH1 and HRH2. They determined that HRH2 shares 61% amino acid sequence identity with S. cerevisiae JA1. Imamura et al. (1997) isolated cDNAs corresponding to the entire coding region of HRH2, which they called DBP1 (DEAH box protein 1). The predicted 813-amino acid protein contains 7 consecutive motifs characteristic of ATP-dependent RNA helicases, as well as consensus sequences for structural motifs of a DNA/RNA helicase with a DEAH box. Northern blot analysis revealed that DBP1 was expressed as a 3.4-kb mRNA in all tissues tested. An additional larger transcript was observed in many tissues. Gee et al. (1997) isolated cDNAs encoding the mouse HRH2 homolog, mDEAH9. Gee et al. (1997) reported that mDEAH9 and Prp43 are 65% identical over a 500-amino acid region spanning the central helicase domain and C-terminal region, and that mDEAH9 and HRH2 were 98% identical in the helicase domain. When expressed in yeast, mDEAH9 complemented the Prp43 mutation specifically, although with less efficiency than the native yeast protein. Immunofluorescence experiments showed that mDEAH9 colocalizes with splicing factor SC35 (600813) in punctate nuclear speckles in mammalian cells, consistent with its predicted role as a pre-mRNA splicing factor. Gee et al. (1997) suggested that mDEAH9 represents a mammalian homolog of Prp43.MAPPING
By fluorescence in situ hybridization, Imamura et al. (1997) mapped the DHX15 gene to chromosome 4p15.3. ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 603403 was added.