Cytochrome b5 type B (CYB5B)

The protein contains 146 amino acids for an estimated molecular weight of 16332 Da.

 

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases. (updated: May 8, 2019)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

This protein is predicted to be membranous by TOPCONS.

Topcons

Interpro domains
Total structural coverage: 100%
Model score: 27
MODL_MODELLER-9.18
MODL_I-TASSER-3.0

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The reference OMIM entry for this protein is 611964

Cytochrome b5, type b (outer mitochondrial membrane); cyb5b
Outer mitochondrial membrane cytochrome b5; omb5
Cypb5m
Cytb5, type 2

CLONING

By PCR of human placenta, liver, and testis cDNA libraries, followed by database analysis, Soucy and Luu-The (2002) isolated CYB5B. The deduced 153-amino acid protein shares 45.8% homology with CYB5A (613218), and RT-PCR analysis showed CYB5B expression in human liver, testis, and adrenal tissues. CYB5B had 17,20-lyase activity as measured by the conversion of pregnenolone to dehydroepiandrosterone (DHEA) when expressed in HEK293 cells.

MAPPING

Hartz (2010) mapped the CYB5B gene to chromosome 16q22.1 based on an alignment of the CYB5B sequence (GenBank GENBANK AB009282) with the genomic sequence (GRCh37). ... More on the omim web site

Subscribe to this protein entry history

May 11, 2019: Protein entry updated
Automatic update: Entry updated from uniprot information.

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

June 20, 2017: Protein entry updated
Automatic update: comparative model was added.

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 611964 was added.

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed