TBC1 domain family member 10B (TBC1D10B)
The protein contains 808 amino acids for an estimated molecular weight of 87199 Da.
Acts as GTPase-activating protein for RAB3A, RAB22A, RAB27A, AND RAB35. Does not act on RAB2A and RAB6A. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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No binding partner found
Biological Process
Activation of GTPase activity
Intracellular protein transport
Regulation of GTPase activity
Regulation of vesicle fusion
Retrograde transport, endosome to Golgi
Cellular Component
Cytoplasm
Cytosol
Endomembrane system
Intracellular anatomical structure
Plasma membrane
The reference OMIM entry for this protein is 613620
Tbc1 domain family, member 10b; tbc1d10b
Rab27agap-beta
DESCRIPTION
Small G proteins of the RAB family (see 179508) function in intracellular vesicle trafficking by switching from the GTP-bound state to the GDP-bound state with the assistance of guanine nucleotide exchange factors (GEFs; see 609700) and GTPase-activating proteins (GAPs). TBC1D10B functions as a GAP for several proteins of the Rab family (Ishibashi et al., 2009).CLONING
By database analysis, Itoh and Fukuda (2006) determined that the deduced 533-amino acid TBC1D10B protein, which they called RAB27AGAP-beta, contains a central TRE2 (USP6; 604334)/yeast Bub2/CDC16 (603461) (TBC) domain and a C-terminal DAYF motif. The TBC domain is predicted to function as a RabGAP, and the DAYF motif is predicted to interact with PDZ domains. TBC1D10B shares highest sequence similarity with EPI64 (TBC1D10A; 610020). The TBC domains of TBC1D10B and TBC1D10A are 81% identical, and both contain a highly conserved catalytic arginine. Fluorescence-tagged TBC1D10B localized to the plasma membrane of transfected mouse melanoma cells. Ishibashi et al. (2009) found that fluorescence-tagged TBC1D10B localized near the plasma membrane of differentiated rat PC12 pheochromocytoma cells.GENE FUNCTION
By screening human and mouse cDNAs encoding putative RabGAPs for the ability to catalyze GTP exchange in Rab proteins, Itoh and Fukuda (2006) found that both TBC1D10A and TBC1D10B functioned as GAPs for mouse Rab27a (603868) and induced melanosome aggregation in a mouse melanocyte cell line. Both TBC1D10A and TBC1D10B increased the GTPase activity of Rab27a and reduced the amount of GTP-Rab27a when coexpressed with Rab27a. Endogenous Rab3a (179490) is present on dense-core vesicles in the distal portion of neurites in differentiated rat PC12 cells. By overexpressing TBC1D10B in PC12 cells, Ishibashi et al. (2009) found that TBC1D10B functioned as a GAP for Rab3a, reducing association of Rab3a with neurites. Mutation of the catalytic arg134 in the TBC domain of TBC1D10B completely inhibited its GAP activity against Rab3a in PC12 and transfected COS-7 cells. TBC1D10B also showed significant GAP activity toward Rab22a (612966), Rab27a, and Rab35 (604199), but not Rab2a (RAB2; 179509) or Rab6a (179513).MAPPING
Hartz (2010) mapped the TBC1D10B gene to chromosome 16p11.2 based on an alignment of the TBC1D10B sequence (GenBank GENBANK BC063112) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 613620 was added.