Acidic fibroblast growth factor intracellular-binding protein (FIBP)
The protein contains 364 amino acids for an estimated molecular weight of 41878 Da.
May be involved in mitogenic function of FGF1. May mediate with IER2 FGF-signaling in the establishment of laterality in the embryo (By similarity). (updated: Oct. 25, 2017)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs11559154 | |
| dbSNP:rs2231893 | |
| dbSNP:rs36080962 |
Cellular Component
Endomembrane system
Extracellular exosome
Membrane
Mitochondrion
Nuclear speck
Nucleoplasm
Nucleus
The reference OMIM entry for this protein is 608296
Fibroblast growth factor, acidic, intracellular binding protein; fibp
CLONING
Using the yeast 2-hybrid system, Kolpakova et al. (1998) identified a HeLa cell cDNA, which the authors termed FIBP, that interacts with acidic fibroblast growth factor (FGF1; 131220). The FIBP cDNA encodes a deduced 372-amino acid protein with a predicted molecular mass of 42 kD. The protein is mainly hydrophilic and has no N-terminal signal sequence. Northern blot analysis demonstrated that FIBP and FGF1 have similar expression patterns. FIBP is expressed ubiquitously as an approximately 1.4-kb transcript at highest levels in heart, skeletal muscle, and pancreas, and at somewhat lower levels in brain. In vitro experiments showed that FIBP readily attaches to microsomal membranes and is capable of binding FGF1 with high affinity. Immunofluorescence studies demonstrated that FIBP is mainly localized in the nucleus and to a lesser extent in mitochondria and other cytoplasmic membranes. Western blot analysis indicated the presence of 2 forms of FIBP. By RT-PCR analysis, Kolpakova et al. (2000) identified a second FIBP transcript caused by alternative splicing of exon 5 and resulting in a protein with an additional 7 residues. They cloned and characterized the mouse Fibp gene and, by database searching, identified a Drosophila homolog. Human FIBP shares 97% and 46% sequence identity with the mouse and Drosophila homologs, respectively.GENE STRUCTURE
Kolpakova et al. (2000) determined that the FIBP gene contains 10 exons and spans more than 5 kb. Sequence analysis of the region surrounding the translation start revealed a CpG island. Functional studies of the promoter region suggested strong transcriptional activity.MAPPING
By FISH, Kolpakova et al. (2000) mapped the FIBP gene to chromosome 11q13.1. ... More on the omim web site
Feb. 5, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 15, 2016: Protein entry updated
Automatic update: OMIM entry 608296 was added.
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed