Keratinocyte proline-rich protein (KPRP)
The protein contains 579 amino acids for an estimated molecular weight of 64136 Da.
No function (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs17612167 | |
| dbSNP:rs944683 | |
| dbSNP:rs16834457 | |
| dbSNP:rs16834461 | |
| dbSNP:rs4329520 | |
| dbSNP:rs6703294 |
No binding partner found
The reference OMIM entry for this protein is 613260
Keratinocyte proline-rich protein; kprp
CLONING
Using suppression subtractive hybridization to identify cDNA clones induced by calcium in normal human epidermal keratinocytes, followed by 5-prime and 3-prime RACE, Lee et al. (2005) cloned KPRP. The deduced 579-amino acid protein has a calculated molecular mass of 64 kD. It has a glutamine-rich N-terminal half, a proline-rich C-terminal half, and 8 putative N-myristoylation sites. Northern blot analysis of mouse tissues detected Kprp expression in stomach and skin. In situ hybridization revealed KPRP expression in the upper granular layer of differentiated normal human skin. In human fetal skin, KPRP expression began in the periderm at 16 weeks' estimated gestational age (EGA), and it was expressed in the granular and horny layers of the epidermis at 24 weeks' EGA and thereafter. In mice, expression began at embryonic day 17.5. Western blot analysis detected KPRP at an apparent molecular mass of 70 kD.GENE FUNCTION
Lee et al. (2005) found that elevated intracellular calcium resulted in a time-dependent increase in KPRP expression in normal human epidermal keratinocytes. Expression of KPRP was elevated in psoriatic lesions compared with normal skin.MAPPING
By genomic sequence analysis, Lee et al. (2005) mapped the KPRP gene to the epidermal differentiation complex on chromosome 1q21. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for KPRP
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 613260 was added.