Kinesin light chain 3 (KLC3)
The protein contains 504 amino acids for an estimated molecular weight of 55364 Da.
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Cellular Component
Ciliary rootlet
Cytoplasm
Kinesin complex
Microtubule
Motile cilium
Neuron projection
The reference OMIM entry for this protein is 601334
Kinesin light chain 3; klc3
Kinesin light chain 2-like; klc2l
Kns2b
CLONING
Lamerdin et al. (1996) reported the comparative analysis of 91.6 kb of sequence including 54.3 kb encompassing the human ERCC2 locus (126340) on 19q13.2-q13.3, the syntenic region in the mouse (32.6 kb), and a further 4.7 kb of sequence 3-prime of the previously reported ERCC2 region in the hamster. In addition to ERCC2, the analysis revealed the presence of 2 previously undescribed genes, one of which was KLC3, in all 3 species. KLC3, which Lamerdin et al. (1996) had designated KLC2, was most similar to the kinesin light chain gene in sea urchin. The deduced human protein contains 504 amino acids and shows significant sequence identity with the human KLC1 protein (600025).GENE STRUCTURE
Lamerdin et al. (1996) found by sequence comparison that the human, mouse, and hamster KLC3 genes share 12 conserved exons; the human gene contains 10 (as well as 1 alternate exon), the mouse contains 11, and the hamster 8.MAPPING
By sequence analysis, Lamerdin et al. (1996) identified the KLC3 gene on chromosome 19q13.2-q13.3. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for KLC3
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 601334 was added.