Ubiquitin-conjugating enzyme E2 R2 (UBE2R2)

The protein contains 238 amino acids for an estimated molecular weight of 27166 Da.

 

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. May be involved in degradation of katenin. (updated: April 1, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 81%
Model score: 87

(right-click above to access to more options from the contextual menu)

The reference OMIM entry for this protein is 612506

Ubiquitin-conjugating enzyme e2r 2; ube2r2
Ubiquitin-conjugating enzyme ubc3b; ubc3b

CLONING

Using the casein kinase II (CK2) catalytic subunits alpha (CSNK2A1; 115440) and alpha-prime (CSNK2A2; 115442) as bait in a yeast 2-hybrid screen of human and mouse cDNA libraries, Semplici et al. (2002) cloned the full-length cDNAs corresponding to the UBE2R2 gene, which they designated UBC3B, in both species. Both the human and mouse deduced UBC3B proteins contain 238 amino acids. UBC3B is highly homologous to UBC3 (CDC34; 116948), with only 47 amino acid differences, and has the conserved cysteine and leucine residues that are part of the active site in ubiquitin-conjugating enzymes (E2). The UBC3B protein shares 35% sequence identity with S. cerevisiae cdc34.

GENE FUNCTION

Semplici et al. (2002) showed that human UBC3B complemented a yeast cdc34 temperature-sensitive mutant. Deletion and site-directed mutagenesis demonstrated that CK2 phosphorylated UBC3B in the C-terminal domain at serine-233; mutagenesis of the corresponding serine residue at position 231 of human CDC34/UBC3 showed that this was the major CK2-phosphorylation site in this protein. In vitro binding experiments demonstrated that phosphorylated UBC3B and CDC34 bound specifically to the F-box protein beta-TRCP (BTRC; 603482), which resulted in enhanced degradation of beta-catenin (116806), a substrate of BTRC. Semplici et al. (2002) suggested that CK2-dependent phosphorylation of CDC34 and UBC3B functions by regulating BTRC substrate recognition.

MAPPING

Semplici et al. (2002) stated that the UBC3B gene maps to chromosome 9p22.1. ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

June 20, 2017: Protein entry updated
Automatic update: comparative model was added.

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 612506 was added.