FAD synthase (FLAD1)
The protein contains 587 amino acids for an estimated molecular weight of 65266 Da.
Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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| Variant | Description |
|---|---|
| dbSNP:rs773925274 | |
| LSMFLAD |
The reference OMIM entry for this protein is 610595
Flavin adenine dinucleotide synthetase, s. cerevisiae, homolog of; flad1
Fad1, s. cerevisiae, homolog of
Fad synthetase; fads
Fmn adenylyltransferase
DESCRIPTION
FAD synthetase (EC 2.7.7.2) catalyzes the adenylation of flavin mononucleotide (FMN) into the redox cofactor FAD.CLONING
Wu et al. (1995) cloned the gene for FAD synthetase, which they designated Fad1, in S. cerevisiae. By database analysis with yeast Fad1 as query, Brizio et al. (2006) identified the human homolog, FLAD1. FLAD1 encodes 2 alternatively spliced transcripts, which the authors named FADS1 and FADS2. FADS1 encodes a 587-amino acid protein with a predicted molecular mass of 65.3 kD, and FADS2 encodes a 490-amino acid protein with a predicted molecular mass of 54.2 kD. FADS2 uses a downstream start codon and thus lacks an N-terminal region present in FADS1. Analysis using protein prediction programs showed that both FLAD1 isoforms have a predicted cytoplasmic location, although FADS1 also contains an N-terminal mitochondrial targeting sequence.GENE FUNCTION
Using spectrophotometric and chromatographic measurements of in vitro enzymatic assays of recombinant FLAD1 proteins, Brizio et al. (2006) showed that both FLAD1 isoforms possess MgCl2-dependent FAD synthetase activity.MAPPING
By genomic sequence analysis, Brizio et al. (2006) mapped the FLAD1 gene to chromosome 1. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 610595 was added.