Serine/threonine-protein phosphatase 4 regulatory subunit 1 (PPP4R1)
The protein contains 950 amino acids for an estimated molecular weight of 107004 Da.
Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. (updated: Jan. 7, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs1056191 | |
| dbSNP:rs329003 | |
| dbSNP:rs2306134 | |
| dbSNP:rs2306134 |
The reference OMIM entry for this protein is 604908
Protein phosphatase 4, regulatory subunit 1; ppp4r1
Pp4r1
DESCRIPTION
PPP4R1 is a regulatory subunit of protein phosphatase-4 (PPP4), a protein serine/threonine phosphatase (Kloeker and Wadzinski, 1999).CLONING
By peptide microsequencing of bovine PP4R1 and EST database searching, Kloeker and Wadzinski (1999) identified a human PPP4R1 cDNA clone encoding a 933-amino acid protein. Sequence analysis identified 13 'heat' repeats that are similar to those in the regulatory component PP2A-A of PPP2R1B but are not contiguous. Mass spectrometry analysis indicated that PPP4R1 is a 105-kD protein, whereas SDS-PAGE analysis showed a doublet migrating at 120 to 125 kD. Immunoblot analysis revealed that PPP4R1 copurifies with PPP4C (602035), suggesting that the PPP4 complex contains at least 1 catalytic and 1 regulatory subunit.MAPPING
The International Radiation Hybrid Mapping consortium mapped the PPP4R1 gene to chromosome 18 (TMAP WI-13171). ... More on the omim web site
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 604908 was added.