V-type proton ATPase 116 kDa subunit a1 (ATP6V0A1)

The protein contains 837 amino acids for an estimated molecular weight of 96413 Da.

 

Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity). (updated: June 17, 2020)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 4%
Model score: 36

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The reference OMIM entry for this protein is 192130

Atpase, h+ transporting, lysosomal, v0 subunit a1; atp6v0a1
Atpase, h+ transporting, lysosomal, noncatalytic accessory protein 1a; atp6n1a
Atp6n1
Vacuolar proton pump, subunit 1; vpp1

DESCRIPTION

ATP-driven proton pumps associated with the clathrin-coated vesicles and synaptic vesicles are a group of polypeptides involved in basic cellular processes through acidification of intracellular organelles. These functions include intracellular targeting of enzymes to lysosomes and secretory granules, and receptor-ligand dissociation in receptor-mediated endocytosis (summary by Ozcelik et al., 1991).

MAPPING

Using a rat cDNA clone for the 116-kD subunit of the vacuolar proton pump, Ozcelik et al. (1991) mapped the gene to human chromosome 17 by study of rodent-human hybrid cell lines and the homologous gene to mouse chromosome 11 by a study of Chinese hamster or rat/mouse hybrid cell lines. In hybrid cell lines with fragments of chromosome 17, the VPP1 gene was shown to be located in region 17q21-qter. In the course of constructing a transcription map of approximately 600 kb of genomic DNA surrounding the BRCA1 (113705) gene, Brody et al. (1995) identified the VPP1 gene, thus regionalizing it to 17q21. ... More on the omim web site

Subscribe to this protein entry history

June 29, 2020: Protein entry updated
Automatic update: Entry updated from uniprot information.

Dec. 10, 2018: Protein entry updated
Automatic update: model status changed

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 192130 was added.