Myeloid-derived growth factor (MYDGF)

The protein contains 173 amino acids for an estimated molecular weight of 18795 Da.

 

Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (By similarity). Involved in endothelial cell proliferation and angiogenesis (PubMed:25581518). (updated: April 1, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

Interpro domains
Total structural coverage: 0%
Model score: 0
MODL_ROSETTA-3.4

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VariantDescription
dbSNP:rs2270090

The reference OMIM entry for this protein is 606746

Chromosome 19 open reading frame 10; c19orf10
Interleukin 25; il25
Stroma-derived growth factor 20; sf20

CLONING

By profiling proteins secreted from preadipocytes (mouse 3T3-L1 cells) by means of 2-dimensional electrophoresis mass spectrometry, Wang et al. (2004) identified c19orf10 (SF10; IL25), which was upregulated during differentiation of adipocytes, leading to the suggestion that it is involved in adipogenesis. During a proteomic analysis of fibroblast-like synoviocytes (FLSs), Weiler et al. (2007) identified C19ORF10, which was produced in significant amounts by these cells. The deduced 173-amino acid protein contains 2 O-glycosylation sites and several potential phosphorylation sites. There was evidence of 2 additional splice variants. The human and mouse C19ORF10 proteins share 91% sequence identity. Immunohistochemistry analyses of recombinant C19ORF10 in cultured FLSs and in synovial tissue sections from patients with rheumatoid arthritis (RA) or osteoarthritis (OA) revealed intracellular staining consistent with localization in the endoplasmic reticulum/Golgi apparatus. Sections of RA and OA synovia expressed similar patterns of C19ORF10 distribution with perivascular and synovial lining staining. Double-staining in situ analysis suggested that fibroblast-like synovial cells produced C19ORF10, whereas macrophages, B cells, or T cells produced little or none of this protein.

GENE STRUCTURE

By surveying available cDNA clones, Weiler et al. (2007) found evidence of 3 splice variants of C19ORF10. The most common variant, C19ORF10b, has 6 exons and was supported by sequence data from 468 clones. The other 2 predicted variants, a and c, were supported by 1 clone each.

MAPPING

By database analysis, Weiler et al. (2007) mapped the C29ORF10 gene to chromosome 19p13.3.

HISTORY

Tulin et al. (2001) reported the cloning of full-length cDNAs encoding mouse and human SF20, which they also referred to as interleukin-25 (IL25). However, they retracted their paper in 2003. ... More on the omim web site

Subscribe to this protein entry history

May 12, 2019: Protein entry updated
Automatic update: model status changed

Nov. 17, 2018: Protein entry updated
Automatic update: model status changed

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

Oct. 27, 2017: Protein entry updated
Automatic update: model status changed

March 25, 2017: Additional information
No protein expression data in P. Mayeux work for MYDGF

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 606746 was added.

Feb. 25, 2016: Protein entry updated
Automatic update: model status changed

Feb. 24, 2016: Protein entry updated
Automatic update: model status changed

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed