Protein lifeguard 3 (TMBIM1)

The protein contains 311 amino acids for an estimated molecular weight of 34607 Da.

 

Negatively regulates aortic matrix metalloproteinase-9 (MMP9) production and may play a protective role in vascular remodeling. (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 97%
Model score: 0

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VariantDescription
dbSNP:rs2292553

No binding partner found

The reference OMIM entry for this protein is 610364

Transmembrane bax inhibitor motif-containing protein 1; tmbim1
Responsive to centrifugal force and shear stress 1; recs1

CLONING

Using cDNA subtractive hybridization in human umbilical vein endothelial cells (HUVEC) to search for genes upregulated by shear stress, Yoshisue et al. (2002) cloned TMBIM1, which they called RECS1. Zhao et al. (2006) determined that the 311-amino acid protein has a calculated molecular mass of 34.6 kD. Western blot analysis of HUVEC and smooth muscle cells detected RECS1 at an apparent molecular mass of 30 kD. Fluorescence-tagged RECS1 was expressed in endosomal and lysosomal membranes. Zhao et al. (2006) cloned mouse Tmbim1. The 309-amino acid protein has a 100-amino acid N-terminal proline- and glycine-rich domain followed by 7 transmembrane domains. The N-terminal domain also contains a PPxY motif within a potential PEST sequence and has several PxxP motifs. There is a minimal consensus SUMO acceptor site between transmembrane domains 4 and 5. Northern blot analysis detected mouse Recs1 in most tissues except for thymus, spleen, and testis. RT-PCR also detected Recs1 in murine fibroblasts and white blood cells.

GENE FUNCTION

Zhao et al. (2006) showed that Recs1-null mouse aortic smooth muscle cells had higher levels of active and pro-Mmp9 (120361) compared to wildtype cells, but Mmp2 (120360) protein levels were equivalent in these cells. They suggested that RECS1 is a negative regulator of aortic MMP9 production.

GENE STRUCTURE

Zhao et al. (2006) determined that the mouse Tmbim1 gene contains 11 exons and spans about 7.2 kb.

MAPPING

The International Radiation Hybrid Mapping Consortium mapped the TMBIM1 gene to chromosome 2 (TMAP RH12007).

ANIMAL MODEL

Zhao et al. (2006) found Recs1-knockout mice were born at an expected mendelian frequency, were fertile, and appeared normal. However, mice over 14 months of age developed cystic medial degeneration (CMD) as evidenced by overt thoracic aortic media degeneration and acidic mucopolysaccharide deposition. Lesions were not associated with inflammatory changes. Using Western blot analysis and gelatin zymography, Zhao et al. (2006) found that CMD and aortic dilation in aged Recs1-knockout mice were associated with increased levels of active Mmp9 and Mmp2. ... More on the omim web site

Subscribe to this protein entry history

May 12, 2019: Protein entry updated
Automatic update: model status changed

Nov. 17, 2018: Protein entry updated
Automatic update: model status changed

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Oct. 27, 2017: Protein entry updated
Automatic update: model status changed

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 610364 was added.

Feb. 25, 2016: Protein entry updated
Automatic update: model status changed

Feb. 24, 2016: Protein entry updated
Automatic update: model status changed

Jan. 25, 2016: Protein entry updated
Automatic update: model status changed