Receptor expression-enhancing protein 6 (REEP6)
The protein contains 184 amino acids for an estimated molecular weight of 20733 Da.
Required for correct function and survival of retinal photoreceptors (PubMed:27889058). Required for retinal development (By similarity). In rod photoreceptors, facilitates stability and/or trafficking of guanylate cyclases and is required to maintain endoplasmic reticulum and mitochondrial homeostasis (By similarity). May play a role in clathrin-coated intracellular vesicle trafficking of proteins from the endoplasmic reticulum to the retinal rod plasma membrane (By similarity). (updated: May 8, 2019)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs2271412 | |
| RP77 | |
| RP77 | |
| RP77 |
Biological Process
Detection of light stimulus involved in visual perception
Endoplasmic reticulum organization
Endoplasmic reticulum tubular network organization
Regulation of intracellular transport
Cellular Component
Apical part of cell
Clathrin-coated vesicle membrane
Endoplasmic reticulum
Endoplasmic reticulum membrane
Endoplasmic reticulum tubular network
Integral component of membrane
Membrane
Nucleus
Photoreceptor inner segment
Rod spherule
Molecular Function
The reference OMIM entry for this protein is 609346
Receptor expression-enhancing protein 6; reep6
Deleted in polyposis 1-like 1; dp1l1
Tb2-like 1; tb2l1
Chromosome 19 open reading frame 32; c19orf32
CLONING
Transport of G protein-coupled receptors (GPCRs) to the cell surface membrane is critical for receptor-ligand recognition. However, mammalian GPCR odorant receptors (ORs), when heterologously expressed in cells, are poorly expressed on the cell surface. By screening for genes that induced cell surface expression of ORs expressed in human embryonic kidney cells, Saito et al. (2004) identified mouse and human REEP1 (609139). They searched databases for homologs of REEP1 and identified several other REEP genes, including REEP6. In situ hybridization of mouse olfactory epithelium revealed expression of Reep6 in supporting cells, but not in olfactory neurons. By subtractive hybridization to identify novel mouse genes expressed in retina, Sato et al. (2005) cloned Dp1l1. The deduced 201-amino acid protein shares 57% identity with mouse and human DP1 (DP1; 125265). Northern blot analysis detected high expression of Dp1l1 in mouse retina and liver, with lower levels in kidney and testis, and RT-PCR showed low expression in spleen and lung. No expression was detected in brain. In situ hybridization and immunohistochemical analyses showed that Dp1l1 mRNA and protein were localized richly in retinal ganglion cells (RGCs). Dp1l1 was present in the cytoplasm in a punctate pattern. Sato et al. (2005) concluded that Dp1l1 is a membrane protein that likely plays a role in intracellular membrane trafficking in RGCs.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the REEP6 gene to chromosome 19 (TMAP RH92355). By radiation hybrid analysis, Sato et al. (2005) mapped the mouse Dp1l1 gene to chromosome 10 in a region that shows homology of synteny to human chromosome 19p13.3. ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: model status changed
May 11, 2019: Protein entry updated
Automatic update: Entry updated from uniprot information.
Nov. 17, 2018: Protein entry updated
Automatic update: model status changed
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 27, 2017: Protein entry updated
Automatic update: model status changed
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 609346 was added.
Feb. 25, 2016: Protein entry updated
Automatic update: model status changed
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed