Condensin complex subunit 3 (NCAPG)
The protein contains 1015 amino acids for an estimated molecular weight of 114334 Da.
Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. (updated: Feb. 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs35722563 | |
| a colorectal cancer sample; somatic mutation | |
| dbSNP:rs3795243 |
Cellular Component
Actin cytoskeleton
Centrosome
Condensed chromosome
Condensed chromosome, centromeric region
Condensin complex
Cytoplasm
Cytosol
Membrane
Nucleus
Molecular Function
The reference OMIM entry for this protein is 606280
Non-smc condensin i complex subunit g; ncapg
Condensin i complex, non-smc subunit g
Chromosome-associated protein g; capg
Members of the structural maintenance of chromosomes (SMC) family of proteins (e.g., CAPC; 605575) are critical for mitotic chromosome condensation in frogs and for DNA repair in mammals.
CLONING
Jager et al. (2000) screened a melanoma cDNA library by SEREX (serologic analysis of cDNA expression libraries) and isolated a novel cDNA, called NY-MEL-3, encoding a deduced 1,015-amino acid mitotic protein highly homologous to the Xenopus chromosome condensation protein XCAP-G and designated human CAPG. RT-PCR and Northern blot analyses showed highest expression of CAPG in the testis among normal tissues and variable expression in tumor cells, reflecting the proliferative activity in these cells.GENE FUNCTION
In contrast to most genomic DNA in mitotic cells, the promoter regions of some genes, such as the stress-inducible hsp70i gene that codes for a heat-shock protein, remain uncompacted, a phenomenon known as bookmarking. Xing et al. (2005) showed that hsp70i (see 140550) bookmarking is mediated by a transcription factor called HSF2 (140581), which binds this promoter in mitotic cells, recruits protein phosphatase 2A (see 605997), and interacts with the CAPG subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the CAPG gene to chromosome 4 (TMAP stSG54871). ... More on the omim web site
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 606280 was added.