Serine/threonine-protein phosphatase CPPED1 (CPPED1)

The protein contains 314 amino acids for an estimated molecular weight of 35548 Da.

 

Protein phosphatase that dephosphorylates AKT family kinase specifically at 'Ser-473', blocking cell cycle progression and promoting cell apoptosis. May play an inhibitory role in glucose uptake by adipocytes. (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology.


Interpro domains
Total structural coverage: 0%
Model score: 0
No model available.

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VariantDescription
dbSNP:rs3748976
dbSNP:rs3748980
dbSNP:rs1713480
dbSNP:rs11645068

The reference OMIM entry for this protein is 615603

Calcineurin-like phosphoesterase domain-containing protein 1; cpped1

GENE FUNCTION

Using quantitative RT-PCR, Vaittinen et al. (2013) confirmed that expression of CPPED1 in subcutaneous adipose tissue was reduced at 8 months following weight reduction compared with weight-maintenance controls. Expression of CPPED1 was highest in human SGBS (312870) preadipocytes, was downregulated in culture, and did not change with differentiation. Knockdown of CPPED1 in SGBS adipocytes increased mRNA expression of ADIPOQ (605441), ADIPOR1 (607945), and GLUT4 (SLC2A4; 138190), decreased mRNA expression of GLUT1 (SLC2A1; 138140) and LEP (164160), and increased secretion of high molecular mass adiponectin. CPPED1 knockdown also increased insulin (176730)-stimulated glucose uptake, which was abolished by treatment with wortmannin, a PI3 kinase (see 601232) inhibitor.

MAPPING

Hartz (2014) mapped the CPPED1 gene to chromosome 16p13.12 based on an alignment of the CPPED1 sequence (GenBank GENBANK AK225359) with the genomic sequence (GRCh37). ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 615603 was added.