Ubiquitin-related modifier 1 (URM1)

The protein contains 101 amino acids for an estimated molecular weight of 11380 Da.

 

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates. (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 100%
Model score: 44

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The reference OMIM entry for this protein is 612693

Ubiquitin-related modifier 1, s. cerevisiae, homolog of; urm1

CLONING

By searching databases for sequences similar to mouse Urm1, Singh et al. (2005) identified human URM1. The deduced 101-amino acid protein shares 93% identity with mouse Urm1. Schlieker et al. (2008) cloned human URM1 from a HeLa cell cDNA library.

BIOCHEMICAL FEATURES

Using nuclear magnetic resonance spectroscopy, Singh et al. (2005) determined the solution structure of mouse Urm1. They found that Urm1 assumed a beta-grasp fold similar to those of eukaryotic ubiquitin (UBB; 191339) and the bacterial sulfur carrier proteins MoaD and ThiS.

GENE FUNCTION

Schlieker et al. (2008) showed that knockdown of URM1 via short hairpin RNA reduced the growth rate of HeLa cells. Knockdown of URM1 in human cell lines increased cell size, caused multinucleation, and increased the population of cells with 4n or more chromosomes, suggesting that URM1 is required for cytokinesis and thus for orderly cell cycle progression. Use of a URM1-dependent suicide inhibitor with HEK293 cells showed that URM1 interacted with ATPBD3 (612694). Biochemical analysis revealed that the C-terminal thiocarboxylated glycine of URM1 functioned as a sulfur carrier for the thiolation of tRNAs by ATPBD3. Knockdown of either URM1 or ATPBD3 in HeLa cells reduced thiolation of uracil in the anticodon region of tRNA-lys(UUU) (see TRNAK1; 189918).

MAPPING

Hartz (2009) mapped the URM1 gene to chromosome 9q34.11 based on an alignment of the URM1 sequence (GenBank GENBANK AK001880) with the genomic sequence (build 36.1). ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

June 20, 2017: Protein entry updated
Automatic update: comparative model was added.

March 25, 2017: Additional information
No protein expression data in P. Mayeux work for URM1

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 612693 was added.