UBX domain-containing protein 6 (UBXN6)

The protein contains 441 amino acids for an estimated molecular weight of 49754 Da.

 

May negatively regulate the ATPase activity of VCP, an ATP-driven segregase that associates with different cofactors to control a wide variety of cellular processes (PubMed:26475856). As a cofactor of VCP, it may play a role in the transport of CAV1 to lysosomes for degradation (PubMed:21822278, PubMed:23335559). It may also play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins (PubMed:19275885). Together with VCP and other cofactors, it may play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes (PubMed:27753622). (updated: Nov. 22, 2017)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

Interpro domains
Total structural coverage: 0%
Model score: 42
MODL_ROSETTA-3.4

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VariantDescription
dbSNP:rs1127888
dbSNP:rs35436704

The reference OMIM entry for this protein is 611946

Ubx domain protein 6; ubxn6
Ubx domain-containing gene 1; ubxd1

CLONING

By database analysis, Carim-Todd et al. (2001) identified UBXD1. They isolated 2 transcripts encoding predicting proteins of 388 and 441 amino acids with calculated molecular masses of 43.8 and 49.7 kD, respectively. UBXD1 contains a C-terminal UBX domain, characteristic of ubiquitin regulatory proteins, and shares 80% and 79% amino acid identity with the mouse and rat homologs, respectively. Northern blot analysis of adult human tissues detected 1.6-, 1.8-, and 5.5-kb transcripts with highest expression in testis, followed by prostate, spleen, thymus, small intestine, colon, blood leukocytes, and uterus.

GENE STRUCTURE

Carim-Todd et al. (2001) determined that the UBXD1 gene contains 11 exons with an alternatively spliced exon 1a. The 1.8-kb transcript contains an in-frame stop codon in exon 1a preceding a second open reading frame and results in the shorter isoform.

MAPPING

By genomic sequence analysis, Carim-Todd et al. (2001) mapped the UBXD1 gene to chromosome 19p13. ... More on the omim web site

Subscribe to this protein entry history

Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 611946 was added.

Feb. 25, 2016: Protein entry updated
Automatic update: model status changed

Feb. 24, 2016: Protein entry updated
Automatic update: model status changed

Jan. 25, 2016: Protein entry updated
Automatic update: model status changed