STE20-related kinase adapter protein beta (STRADB)
The protein contains 418 amino acids for an estimated molecular weight of 47026 Da.
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation (By similarity). (updated: Feb. 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| a metastatic melanoma sample; somatic mutation | |
| dbSNP:rs35636836 |
Biological Process
Activation of protein kinase activity
Cell cycle arrest
Cell morphogenesis
Insulin receptor signaling pathway
Intracellular signal transduction
JNK cascade
MAPK cascade
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
Neuron projection morphogenesis
Protein export from nucleus
Protein phosphorylation
Regulation of apoptotic process
Regulation of mitotic cell cycle
Signal transduction by protein phosphorylation
Stress-activated protein kinase signaling cascade
The reference OMIM entry for this protein is 607333
Ste20-related kinase adaptor beta; stradb
Ilp-interacting protein; ilpip
Als2 chromosome region gene 2; als2cr2
CLONING
By sequencing candidate genes in the ALS2 (205100) critical region, Hadano et al. (2001) identified ALS2CR2. The deduced 418-amino acid protein has a calculated molecular mass of about 47 kD. ALS2CR2 contains a typical protein kinase domain and shares significant homology with more than 30 serine/threonine protein kinases. Northern blot analysis revealed highest expression of a 2.4-kb transcript in heart, skeletal muscle, and colon. A 2.6-kb transcript, which may represent use of an alternate polyadenylation signal, was detected in testis. Sanna et al. (2002) cloned ALS2CR2, which they called ILPIP, in a yeast 2-hybrid screen of a fetal brain cDNA library using XIAP (300079) as bait. They isolated the full-length cDNA by screening a liver cDNA library. The 418-amino acid ILPIP protein, which the authors termed ILPIP-alpha, contains a putative N-terminal ATP-binding site, a protein kinase domain, and several C-terminal phosphorylation sites. Sanna et al. (2002) also identified a 280-amino acid isoform, which they termed ILPIP-beta, that lacks the first 138 amino acids of the N terminus of ILPIP-alpha. Northern blot analysis detected expression of a 2.4-kb transcript in muscle, liver, and heart, as well as in embryonic kidney cells.GENE FUNCTION
Sanna et al. (2002) determined that ILPIP potentiates the antiapoptotic activity of XIAP by enhancing XIAP-mediated activation of JNK1 (MAPK8; 601158) and other JNK family members, but not by modulating XIAP-mediated caspase inhibition. They also found that expression of a catalytically inactive TAK1 (MAP3K7; 602614) mutant blocked the XIAP/ILPIP activation of JNK1. In vivo coprecipitation experiments showed that both ILPIP and XIAP interact with TAK1 and TRAF6 (602355). Sanna et al. (2002) concluded that XIAP-mediated protection from apoptosis utilizes both a JNK1 activation pathway that involves ILPIP and a caspase inhibition pathway that is independent of ILPIP.GENE STRUCTURE
Hadano et al. (2001) determined that the ALS2CR2 gene contains 12 exons and spans 30 kb. It is transcribed in the centromere-to-telomere direction. The promoter for ALS2CR2 is close to, or overlaps, the promoter for ALS2CR3 (607334), which is transcribed in the opposite orientation.MAPPING
Hadano et al. (2001) mapped the STRADB gene within the ALS2 critical region on chromosome 2q33-q34. They identified 2 intronless pseudogenes that map to chromosomes 1 and 9. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for STRADB
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 607333 was added.