Ras-related protein Rab-6C (RAB6C)
The protein contains 254 amino acids for an estimated molecular weight of 28355 Da.
May be involved in the regulation of centrosome duplication and cell cycle progression. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs4662674 |
No binding partner found
Biological Process
Cell cycle process
Intra-Golgi vesicle-mediated transport
Intracellular protein transport
Metabolic process
Mitotic cell cycle
Rab protein signal transduction
Regulation of centrosome duplication
Response to drug
Retrograde transport, endosome to Golgi
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
Small GTPase mediated signal transduction
Cellular Component
Centrosome
Cytosol
Endomembrane system
Golgi apparatus
Intracellular anatomical structure
Nucleus
The reference OMIM entry for this protein is 612909
Ras-associated protein rab6c; rab6c
Wth3
CLONING
By methylation-sensitive representational difference analysis, Shan et al. (2002) isolated a DNA fragment of RAB6C that was hypermethylated in a multidrug-resistant MCF7 breast cancer cell line. Using 3-prime RACE of a combined human tissue cDNA library, they obtained full-length RAB6C, which they called WTH3. The deduced 254-amino acid WTH3 protein shares significant homology with 2 isoforms of RAB6A (179513), but it has a C-terminal extension compared with these proteins.GENE FUNCTION
Using quantitative RT-PCR, Shan et al. (2002) found that expression of WTH3 was reduced in multidrug-resistant cancer cell lines compared with the parental cell lines. Reexpression of WTH3 in multidrug-resistant cell lines increased their sensitivity to several anticancer drugs, including doxorubicin. Flow cytometry and fluorescence microscopy suggested that WTH3 stimulated cellular uptake and retention of doxorubicin. Tian et al. (2005) found that the WTH3 promoter region was hypermethylated in multidrug-resistant MCF7 cells compared with the parental cell line. EMSA showed that different pools of nuclear proteins were attracted to the hypermethylated and nonmethylated WTH3 probes. Tian et al. (2005) concluded that expression of WTH3 is controlled by both epigenetic modification and transcription factor modulation. Tian et al. (2007) identified a p53 (TP53; 191170)-binding site in the promoter region of the WTH3 gene and showed that WTH3 was upregulated by p53 in vitro and in vivo. Overexpression of WTH3 promoted an apoptotic phenotype in human cells. Tian et al. (2007) concluded that the antiapoptotic effect of WTH3 is linked to p53 expression.GENE STRUCTURE
Tian et al. (2005) determined that the RAB6C promoter region contains 2 atypical TATA boxes and 2 atypical CAATT boxes. They identified a GC-rich region that includes a CpG island and extends from position -485 relative to the translation initiation site into the 5-prime coding region. The promoter region contains several putative transcription factor-binding sites.MAPPING
Hartz (2009) mapped the RAB6C gene to chromosome 2q21.1 based on an alignment of the RAB6C sequence (GenBank GENBANK AL136727) with the genomic sequence (build 36.1). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for RAB6C
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 612909 was added.