SH3 domain-binding glutamic acid-rich-like protein 3 (SH3BGRL3)
The protein contains 93 amino acids for an estimated molecular weight of 10438 Da.
Could act as a modulator of glutaredoxin biological activity. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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The reference OMIM entry for this protein is 615679
Sh3 domain-binding glutamic acid-rich protein-like protein 3; sh3bgrl3
Sh3bgr-like protein 3
Tnf inhibitory protein b1; tipb1
DESCRIPTION
SH3BGRL3 belongs to the thioredoxin (TXN; 187700) superfamily, but it lacks the CxxC motif essential for catalytic activity (Mazzocco et al., 2001).CLONING
By sequencing peptides from a protein that protected human cell lines from TNF (191160)-induced apoptosis, followed by RT-PCR of TNF-treated HF1 human fibroblasts, Berleth et al. (1999) obtained a partial clone for SH3BGRL3, which they called TIPB1. The predicted protein has regions similar to redox proteins that use glutathione, and it has putative sites for N-myristoylation, phosphorylation, and amidation. Northern blot analysis of several human cell lines detected a major TIPB1 transcript of 1.1 kb. Minor transcripts of about 1.7 and 3.0 kb were detected in some cell lines. Western blot analysis showed a major TIPB1 protein at an apparent molecular mass of 27 kD. By searching an EST database for sequences similar to SH3BGR (602230), Mazzocco et al. (2001) identified SH3BGRL3. The deduced 93-amino acid protein shares 39% identity with the N-terminal region of SH3BGR. It also shares 25% identity with E. coli glutaredoxin (see GLRX, 600443), predominantly in a C-terminal domain. However, SH3BGRL3 lacks both active-site cysteines conserved in glutaredoxin. Northern blot analysis detected SH3BGRL3 in all human tissues and cell lines examined. Fluorescence-tagged SH3BGRL3 was expressed in the nucleus of transfected COS-7 cells.GENE FUNCTION
Berleth et al. (1999) identified TIPB1 among a group of proteins expressed by HF1 human fibroblasts in response to low-dose TNF. TIPB1 protected cells from lysis induced by subsequent high doses of TNF. Purified TIPB1 protected several other human cell lines from apoptosis caused by high-dose TNF, but the degree of protection was cell line dependent.MAPPING
Using FISH, Mazzocco et al. (2001) mapped the SH3BGRL3 gene to chromosome 1p35-p34.3. Gross (2014) mapped the SH3BGRL3 gene to chromosome 1p36.11 based on an alignment of the SH3BGRL3 sequence (GenBank GENBANK AF247790) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 615679 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed