Serine/threonine-protein phosphatase 6 regulatory subunit 2 (PPP6R2)
The protein contains 966 amino acids for an estimated molecular weight of 104942 Da.
Regulatory subunit of protein phosphatase 6 (PP6). May function as a scaffolding PP6 subunit. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs11555194 | |
| dbSNP:rs13057311 |
The reference OMIM entry for this protein is 610877
Saps domain family, member 2; saps2
Protein phosphatase 6, regulatory subunit 2; pp6r2
Kiaa0685
DESCRIPTION
Protein phosphatase regulatory subunits, such as SAPS2, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS2 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; 612725) (Stefansson and Brautigan, 2006).CLONING
By sequencing clones obtained from a size-fractionated brain cDNA library, Ishikawa et al. (1998) cloned SAPS2, which they designated KIAA0685. The deduced 927-amino acid protein shares significant similarity with yeast Sap190. RT-PCR detected strong SAPS2 expression in all tissues examined. By searching databases for sequences similar to yeast Sap155, Sap185, and Sap190, Stefansson and Brautigan (2006) identified SAPS2, which they called PP6R2. The deduced 966-amino acid protein has a calculated molecular mass of 105 kD and contains a central alpha-helical SAPS domain of about 400 amino acids. Northern blot analysis detected 2 transcripts in all tissues examined, with highest expression in testis, followed by liver, heart, and brain.GENE FUNCTION
Using coimmunoprecipitation and protein pull-down assays, Stefansson and Brautigan (2006) showed that epitope-tagged PP6R2 bound endogenous PPP6C, but not PPP2CA (176915). Immunoprecipitates containing PPP6C and PP6R2 showed phosphatase activity against a test protein, and the activity was inhibited by okadaic acid, a serine/threonine phosphatase inhibitor. Stefansson and Brautigan (2006) found that PP6R2 and PPP6C associated with I-kappa-B-epsilon (IKBE, or NFKBIE; 604548) in HeLa cells. PP6R2 did not interact with epitope-tagged IKBA (NFKBIA; 164008) or IKBB (NFKBIB; 604495).MAPPING
By radiation hybrid analysis, Ishikawa et al. (1998) mapped the SAPS2 gene to chromosome 22. ... More on the omim web site
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 610877 was added.