Phospholipid scramblase 4 (PLSCR4)

The protein contains 329 amino acids for an estimated molecular weight of 37005 Da.

 

May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. (updated: April 1, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  4. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  5. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  6. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  7. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 0%
Model score: 22

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VariantDescription
dbSNP:rs3762685
dbSNP:rs1061409

The reference OMIM entry for this protein is 607612

Phospholipid scramblase 4; plscr4

CLONING

Using the sequence of PLSCR1 (604170) as query, Wiedmer et al. (2000) identified an EST containing PLSCR4, and they cloned the full-length cDNA by PCR of a multiple-tissue cDNA library. The deduced 329-amino acid protein contains an N-terminal proline-rich region and a C-terminal Ca(2+)-binding domain. The proline-rich region contains several PxxP motifs that mediate binding to proteins containing SH3 and WW domains. PLSCR4 shares 46% identity with PLSCR1, with most of the identity in the C-terminal region. Northern blot analysis revealed a 4.0-kb transcript expressed at variable levels in all tissues examined except peripheral blood lymphocytes. Compared with PLSCR1, PLSCR2 (607610), and PLSCR3 (607611), PLSR4 was the only member of this family expressed at detectable levels in brain.

MAPPING

By genomic sequence analysis, Wiedmer et al. (2000) mapped the PLSCR4 gene to chromosome 3q23, in a region that also contains the PLSCR1 and PLSCR2 genes. ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 607612 was added.

Jan. 25, 2016: Protein entry updated
Automatic update: model status changed