Ras-related protein Rab-6B (RAB6B)
The protein contains 208 amino acids for an estimated molecular weight of 23462 Da.
Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Intra-Golgi vesicle-mediated transport
Intracellular protein transport
Metabolic process
Protein transport
Rab protein signal transduction
Retrograde transport, endosome to Golgi
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
The reference OMIM entry for this protein is 615852
Ras-associated protein rab6b; rab6b
DESCRIPTION
RAB6B is a member of the large RAB subfamily of small GTPases that play important roles in the regulation of intracellular transport routes (Opdam et al., 2000).CLONING
Using RT-PCR with primers corresponding to conserved RAB domains involved in GTP-binding in colorectal adenocarcinoma cells, Opdam et al. (2000) cloned RAB6B. The deduced 207-amino acid protein is 91% identical to RAB6A (179513), with most differences in the C termini. RAB6B contains 6 conserved motifs involved in GTP-binding and a CxC motif involved in prenylation. Northern blot analysis detected 1.35-, 3.1-, and 5.6-kb transcripts predominantly in human brain and in a human neuroblastoma cell line. Immunoblot analysis showed expression of a 23-kD protein. Immunohistochemical analysis demonstrated expression in perinuclear regions and Golgi apparatus in neuroblastoma cells. In human brain, RAB6B was expressed in microglia, pericytes, and Purkinje cells.GENE FUNCTION
Using GTP-binding assays, Opdam et al. (2000) found that RAB6B had lower binding activity than RAB6A. Both proteins colocalized at the Golgi complex. Yeast 2-hybrid analysis showed that RAB6B, like RAB6A, interacted with KIF20A (605664) and GAPCENA (RABGAP1; 615882). Opdam et al. (2000) concluded that RAB6B exerts a cell-type specific function in neuronal cells. By yeast 2-hybrid, coimmunoprecipitation, and pull-down analyses, Wanschers et al. (2007) showed that RAB6B interacted with bicaudal D1 (BICD1; 602204). Confocal microscopy demonstrated colocalization of RAB6B and BICD1 at the Golgi and vesicles that align along microtubules, and both proteins colocalized with dynein (see 600112) in neurites of neuroblastoma cells. Wanschers et al. (2007) concluded that RAB6B via BICD1 is linked to the dynein/dynactin (see 601143) complex, suggesting a regulatory role for RAB6B in the retrograde transport of cargo in neuronal cells.BIOCHEMICAL FEATURES
Garcia-Saez et al. (2006) determined the crystal structure of RAB6B in its active and inactive forms at 1.8- and 2.3-angstrom resolution, respectively.MAPPING
By radiation hybrid analysis, Opdam et al. (2000) mapped the RAB6B gene to chromosome 3q21-q23. Gross (2014) mapped the RAB6B gene to chromosome 3q22.1 based on an alignment of the RAB6B sequence (GenBank GENBANK AF498940) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for RAB6B
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 615852 was added.