Serine incorporator 1 (SERINC1)

The protein contains 453 amino acids for an estimated molecular weight of 50495 Da.

 

Enhances the incorporation of serine into phosphatidylserine and sphingolipids. (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  4. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 81%
Model score: 21

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VariantDescription
dbSNP:rs13210569
dbSNP:rs13210446
dbSNP:rs17260829

The reference OMIM entry for this protein is 614548

Serine incorporator 1; serinc1
Tms2
Kiaa1253

DESCRIPTION

SERINC1 belongs to the SERINC family of transmembrane proteins that facilitate incorporation of serine into phosphatidylserine and sphingolipids (Inuzuka et al., 2005).

CLONING

By sequencing clones obtained from a size-fractionated adult brain cDNA library, Nagase et al. (1999) cloned SERINC1, which they designated KIAA1253. RT-PCR ELISA detected SERINC1 expression in all adult and fetal tissues and adult brain regions examined. Highest expression was in brain, predominantly in caudate nucleus, and lowest expression was in pancreas, spleen, and testis. Grossman et al. (2000) cloned mouse Serinc1, which they called Tms2, and they identified human TMS2 by database analysis. The deduced mouse and human proteins contain 453 and 440 amino acids, respectively. Both have 11 transmembrane segments, a MYC (190080)-type helix-loop-helix dimerization motif between transmembrane domains 7 and 8, and several putative phosphorylation sites. In situ hybridization of mouse brain revealed Tms2 expression in virtually every neuron, with highest expression in hippocampus, olfactory bulb, cerebral cortex, and granule cell layer of cerebellum. No Tms2 expression was detected in peripheral tissues. Epitope-tagged Tms2 was expressed in a punctate distribution on the surface of transfected HEK293 cells. Inuzuka et al. (2005) found that rat Serinc1 was expressed in endoplasmic reticulum (ER) membranes of transfected COS cells.

GENE FUNCTION

Inuzuka et al. (2005) found that Serinc1 mRNA was upregulated in rat hippocampal CA1-3 neurons following kainate-induced seizure. Expression of rat Serinc1 in E. coli, yeast, or COS cells enhanced the activity of phosphatidylserine synthase (see 612792), resulting in enhanced incorporation of radiolabeled serine into phosphatidylserine. Serinc1 did not enhance serine uptake. In COS cells, Serinc1 also enhanced the activity of serine palmitoyltransferase (see 605712), resulting in incorporation of serine into 3-ketodihydrosphingosine. Serinc1 and serine palmitoyltransferase colocalized at ER membranes, and yeast 2-hybrid experiments revealed that the 2 proteins interacted directly.

MAPPING

Hartz (2012) mapped the SERINC1 gene to chromosome 6q22.31 based on an alignment of the SERINC1 sequence (GenBank GENBANK AB033079) with the genomic sequence (GRCh37). ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

June 20, 2017: Protein entry updated
Automatic update: comparative model was added.

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 614548 was added.