TBC1 domain family member 13 (TBC1D13)

The protein contains 400 amino acids for an estimated molecular weight of 46554 Da.

 

Acts as a GTPase-activating protein for RAB35. Together with RAB35 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes. (updated: Jan. 31, 2018)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 74%
Model score: 32

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VariantDescription
dbSNP:rs1572912

No binding partner found

The reference OMIM entry for this protein is 616218

Tbc1 domain family, member 13; tbc1d13

DESCRIPTION

Rab GTPases (see RAB1, 179508) are small monomeric GTP-binding proteins. TBC1D13 functions as a Rab GTPase-activating protein (GAP) that stimulates GTP hydrolysis by Rab proteins (Davey et al., 2012).

CLONING

Davey et al. (2012) cloned mouse Tbc1d13. The deduced protein is almost entirely composed of a TBC-type GAP domain spanning residues 32 to 400. Tbc1d13 was expressed in a range of tissues and was upregulated with differentiation in 3T3-L1 mouse adipocytes. Immunohistochemical analysis of 3T3-L1 cells revealed that Tbc1d13 was expressed in the cytosol, perinuclear membrane, and punctate structures scattered throughout the cell periphery. Western blot analysis of fractionated 3T3-L1 cells detected Tbc1d13 at an apparent molecular mass of approximately 45 kD in several subcellular compartments, including high-density microsomes, low-density microsomes, and cytosol.

GENE FUNCTION

By overexpression in 3T3-L1 mouse adipocytes, Davey et al. (2012) found that Tbc1d13 inhibited insulin (INS; 176730)-stimulated Glut4 (SLC2A4; 138190) translocation to the plasma membrane. Using yeast 2-hybrid analysis, Davey et al. (2012) found that Tbc1d13 interacted with constitutively active Rab1 and Rab10 (612672); however, Tbc1d13 did not function as a GAP for these GTPases. Instead, Tbc1d13 accelerated GTP hydrolysis by Rab35 (604199). Coexpression of constitutively active Rab35 partially overcame Tbc1d13-dependent inhibition of insulin-stimulated Glut4 translocation.

MAPPING

Hartz (2015) mapped the TBC1D13 gene to chromosome 9q34.11 based on an alignment of the TBC1D13 sequence (GenBank GENBANK AK001605) with the genomic sequence (GRCh38). ... More on the omim web site

Subscribe to this protein entry history

Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.

Feb. 10, 2018: Protein entry updated
Automatic update: OMIM entry 616218 was added.

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

June 20, 2017: Protein entry updated
Automatic update: comparative model was added.