Glucose-induced degradation protein 8 homolog (GID8)
The protein contains 228 amino acids for an estimated molecular weight of 26749 Da.
Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Acts as a positive regulator of Wnt signaling pathway by promoting beta-catenin (CTNNB1) nuclear accumulation (PubMed:28829046). (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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The reference OMIM entry for this protein is 611625
Chromosome 20 open reading frame 11; c20orf11
Two-hybrid-associated protein with ranbpm 1; twa1
CLONING
Using N-terminally truncated RANBPM (RANBP9; 603854) as bait in a yeast 2-hybrid screen of a human lymphocyte cDNA library, followed by PCR, Umeda et al. (2003) cloned C20ORF11, which they called TWA1. The deduced 228-amino acid protein has a calculated molecular mass of 30 kD. TWA1 has an N-terminal LIS1 (PAFAH1B1; 601545) homology (LISH)/C-terminal to LISH (CTLH) motif, which is found in proteins involved in microtubule dynamics, cell migration, nucleokinesis, and chromosome segregation. It also has a C-terminal domain similar to a C-terminal domain in RANBPM. TWA1 shares 99.5% amino acid identity with mouse Twa1 and is well conserved among mammals. Database analysis revealed TWA1 homologs in fly, plant, and yeast. Northern blot analysis showed ubiquitous Twa1 expression in mouse tissues. Western blot analysis detected a 30-kD TWA1 protein in Hela cells and several mammalian cell lines.GENE FUNCTION
Using coimmunoprecipitation analysis, Umeda et al. (2003) showed that endogenous Ranbpm and Twa1 interacted in COS-7 cells. Immunoprecipitation and gel-filtration analyses demonstrated that both proteins interacted with GST-fused human muskelin (MKLN1; 605623) in a 670-kD protein complex in transfected COS-7 cells.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the C20ORF11 gene to chromosome 20 (TMAP RH78524). ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: model status changed
Nov. 17, 2018: Protein entry updated
Automatic update: model status changed
Oct. 2, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 27, 2017: Protein entry updated
Automatic update: model status changed
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 611625 was added.
Feb. 25, 2016: Protein entry updated
Automatic update: model status changed
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed