Dipeptidyl peptidase 3 (DPP3)
The protein contains 737 amino acids for an estimated molecular weight of 82589 Da.
Cleaves and degrades bioactive peptides, including angiotensin, Leu-enkephalin and Met-enkephalin (PubMed:3233187, PubMed:1515063). Also cleaves Arg-Arg-beta-naphthylamide (in vitro) (PubMed:9425109, PubMed:3233187, PubMed:11209758). (updated: Oct. 25, 2017)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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| Variant | Description |
|---|---|
| dbSNP:rs11826683 | |
| dbSNP:rs11550299 | |
| dbSNP:rs2305535 | |
| dbSNP:rs12421620 |
Cellular Component
Cytoplasm
Cytosol
Extracellular exosome
Nuclear speck
Nucleoplasm
Plasma membrane
The reference OMIM entry for this protein is 606818
Dipeptidyl peptidase iii; dpp3
DESCRIPTION
DPP3 (EC 3.4.14.4) is one of the dipeptidyl aminopeptidases that remove N-terminal dipeptides from physiologically active peptides such as angiotensin (see 106150) or enkephalin (131330).CLONING
Fukasawa et al. (1999) isolated a partial DPP3 cDNA clone from a placenta cDNA library, using antibody to the purified protein as probe. By mass spectrometry, Abramic et al. (2000) determined that the molecular mass of full-length DPP3 is 82.5 kD; by SDS-PAGE, the apparent molecular mass is 81.2 kD.GENE FUNCTION
By mutation and enzyme analysis of the rat recombinant DPP3 protein, Fukasawa et al. (1999) found that DPP3 shows zinc-dependent metalloprotease activity and contains a conserved HELLGH motif with similarity to the zincin HExxH consensus motif. Abramic et al. (2000) noted that whereas the enzymes purified from rat and human erythrocytes show the same molecular mass, pI, and enzyme substrates, they differ significantly in their temperature stability, pH optima, kinetic parameters, and sensitivity to divalent cations, sulfhydryl reagents, and microbial protease inhibitors.MAPPING
Fukasawa et al. (2000) mapped the DPP3 gene to chromosome 11q12-q13.1 by FISH. ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 606818 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed