Pleckstrin-2 (PLEK2)
The protein contains 353 amino acids for an estimated molecular weight of 39971 Da.
May help orchestrate cytoskeletal arrangement. Contribute to lamellipodia formation. (updated: Jan. 7, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt.
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| Variant | Description |
|---|---|
| dbSNP:rs34300264 |
The reference OMIM entry for this protein is 608007
Pleckstrin 2; plek2
CLONING
Using the sequence of human pleckstrin (PLEK; 173570) as query, Hu et al. (1999) identified mouse pleckstrin-2, which encodes a deduced 353-amino acid protein. By EST database searching with mouse Plek2, they identified the human homolog, which encodes a deduced protein that is identical to mouse Plek and 39% identical to human PLEK. Like PLEK, human PLEK2 contains an N- and a C-terminal PH domain plus an intervening DEP domain. Unlike PLEK, which contains 3 phosphorylation sites, PLEK2 contains only 1 site comparable to ser117 in PLEK. Northern blot analysis of human tissues detected ubiquitous expression of a 1.7-kb PLEK2 transcript, with highest expression in thymus, prostate, testis, ovary, small bowel, and large bowel.GENE FUNCTION
Using transfection experiments in COS-7 cells, Hu et al. (1999) determined that, unlike PLEK, PLEK2 is not an efficient substrate of PKC. PLEK2 is bound to the cell membrane, and its PH domains appear to contribute to lamellipodia formation. Overexpression of PLEK2 causes large lamellipodia and peripheral ruffle formation. Hu et al. (1999) suggested that PLEK2 may help orchestrate cytoskeletal arrangement.MAPPING
Gross (2014) mapped the PLEK2 gene to chromosome 14q23.3 based on an alignment of the PLEK2 sequence (GenBank GENBANK AF228603) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 608007 was added.
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed