Syntaxin-18 (STX18)
The protein contains 335 amino acids for an estimated molecular weight of 38674 Da.
Syntaxin that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs13134070 | |
| dbSNP:rs36109375 | |
| dbSNP:rs33952588 |
Biological Process
Endoplasmic reticulum membrane organization
Intracellular protein transport
Membrane fusion
Positive regulation of ER to Golgi vesicle-mediated transport
Positive regulation of organelle assembly
Regulation of Golgi organization
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
The reference OMIM entry for this protein is 606046
Syntaxin 18; stx18
In eukaryotic cells, SNARE proteins (SNAP receptors) mediate the docking and/or fusion of transport vesicles with target membranes. See alpha-SNAP (NAPA; 603215). V-SNARES, found on vesicles, interact with t-SNARES (syntaxins), found on target membranes, in a specific manner.
CLONING
Using a yeast 2-hybrid screen with NAPA cDNA as bait, Hatsuzawa et al. (2000) identified a partial human cDNA encoding a novel syntaxin, which they called syntaxin-18. They isolated a complete cDNA by screening a human brain cDNA library. The deduced 335-amino acid protein contains elements similar to other syntaxins, including predicted coiled-coil regions and a putative transmembrane domain near the C terminus. Syntaxin-18 shares 28% sequence identity with the C. elegans T10H9 predicted gene product, but low sequence similarity to other known syntaxins. Northern blot analysis detected ubiquitous expression of a 1.8-kb STX18 transcript. Using subcellular fractionation and immunofluorescence, Hatsuzawa et al. (2000) showed that syntaxin-18 is principally located in the endoplasmic reticulum.GENE FUNCTION
Based on in vivo overexpression and protein transport studies using epitope-tagged proteins, Hatsuzawa et al. (2000) suggested that syntaxin-18 functions in transport between the endoplasmic reticulum and Golgi. ... More on the omim web site
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 606046 was added.