Vacuolar protein sorting-associated protein 28 homolog (VPS28)

The protein contains 221 amino acids for an estimated molecular weight of 25425 Da.

 

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. (updated: April 1, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
  3. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt.


Interpro domains
Total structural coverage: 46%
Model score: 0
No model available.

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The reference OMIM entry for this protein is 611952

Vacuolar protein sorting 28, yeast, homolog of; vps28

DESCRIPTION

Class E vacuolar protein sorting (VPS) proteins, such as VPS28, are required for appropriate sorting of receptors within the endocytic pathway (Bishop and Woodman, 2001).

CLONING

By searching EST databases for homologs of yeast Vps28, Bishop and Woodman (2001) identified human VPS28 and several invertebrate homologs. The 221-amino acid human protein shares 28% amino acid identity with yeast Vps28. RT-PCR analysis revealed ubiquitous expression of a single VPS28 transcript in human tissues. Immunofluorescence and confocal microscopy showed that VPS28 was exclusively cytosolic.

GENE FUNCTION

Using coimmunoprecipitation and crosslinking analysis, Bishop and Woodman (2001) found that human VPS28 interacted with the coiled-coil C-terminal portion of TSG101 (601387). Expression of ATPase-defective VPS4 (609983) shifted a portion of TSG101 and VPS28 from the cytosol to endosomal vacuoles. Bishop and Woodman (2001) concluded that TSG101 and VPS28 are directly involved in endosomal sorting. Philips et al. (2008) showed that the avirulent Mycobacterium smegmatis species could multiply in Drosophila S2 cells or murine macrophages in which small interfering RNA had disrupted components of the ESCRT (endosomal sorting complex required for transport) complex, including Tsg101 and Vps28, by modulating the phagosome induced by mycobacteria. Immunofluorescence and confocal microscopy showed that Tsg101- or Vps28-depleted cells harbored mycobacteria in a heavily ubiquitinated vacuolar location. Philips et al. (2008) concluded that ESCRT machinery is critical for containment of mycobacterial proliferation.

MAPPING

The International Radiation Hybrid Mapping Consortium mapped the VPS28 gene to chromosome 8 (TMAP A006J21). ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 611952 was added.