Aspartyl aminopeptidase (DNPEP)
The protein contains 475 amino acids for an estimated molecular weight of 52428 Da.
Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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The reference OMIM entry for this protein is 611367
Aspartyl aminopeptidase; dnpep
Aspep; dap
DESCRIPTION
Aspartyl aminopeptidase (EC 3.4.11.21) catalyzes the hydrolysis of N-terminal aspartyl and glutamyl residues (Wilk et al., 1998).CLONING
By database analysis using tryptic peptide sequences from purified rabbit brain cytosol aspartyl aminopeptidase as probe, Wilk et al. (1998) identified and subsequently sequenced human aspartyl aminopeptidase (DNPEP) as well as homologs in mouse and other species. The deduced 490-amino acid human protein has a calculated molecular mass of approximately 52 kD and shares similarity with S. cerevisiae aminopeptidase I, a zinc metalloproteinase found in the vacuole. Sequence analysis of the DNPEP protein in various species revealed several conserved residues including 3 histidines, 3 glutamates, and 5 aspartates. Northern blot analysis of rat tissues detected a 1.9-kb Dnpep transcript in all tissues examined, with highest expression in testis, intermediate expression in kidney and lung, and lesser but significant expression in spleen, liver, and brain.GENE FUNCTION
Using recombinant DNPEP expressed in E. coli, Wilk et al. (1998) showed that human DNPEP catalyzed the dipeptidylpeptidase IV (DPP4; 102720)-dependent hydrolysis of the Asp-Ala bond in the substrate Asp-Ala-Pro-SM at a specific activity of 5.5-47.6 units/mg. DNPEP activity was inhibited by 0.4 mM Zn(2+), was not affected by addition of puromycin, and was significantly reduced when a beta-carboxyl-blocked substrate was substituted.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the DNPEP gene to chromosome 2q (TMAP SHGC-32022). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 611367 was added.
Jan. 27, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed