Syntaxin-8 (STX8)

The protein contains 236 amino acids for an estimated molecular weight of 26907 Da.

 

Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER. (updated: April 1, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

This protein is predicted to be membranous by TOPCONS.

Topcons

Interpro domains
Total structural coverage: 31%
Model score: 46
MODL_ROSETTA-3.4

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The reference OMIM entry for this protein is 604203

Syntaxin 8; stx8

CLONING

Most membrane fusion events in eukaryotic cells are mediated by SNARE proteins on the vesicle (v-SNAREs) and on the target membrane (t-SNAREs or syntaxins). See alpha-SNAP (603215). By searching an EST database, Steegmaier et al. (1998) identified mouse and human cDNAs encoding syntaxin-8. The predicted 236-amino acid human protein shares 23% identity with syntaxin-6 (603944). Like other syntaxins, syntaxin-8 is anchored to membranes by a C-terminal hydrophobic domain and appears to contain a helical domain involved in the formation of a SNARE complex (see SNAP29, 604202). Northern blot analysis revealed that syntaxin-8 was expressed as a 1.3-kb mRNA in all human tissues tested, with the highest levels in heart. When expressed in mammalian cells, syntaxin-8 colocalized with markers of the endoplasmic reticulum (ER).

GENE FUNCTION

Steegmaier et al. (1998) concluded that syntaxin-8 functions as a vesicle trafficking protein in the early secretory pathway, possibly mediating retrograde transport from cis-Golgi membranes to the ER. Independently, Thoreau et al. (1999) isolated syntaxin-8 as a protein that interacted with the regulatory (R) domain of the CFTR (602421) protein in a yeast 2-hybrid screen.

MAPPING

By analysis of somatic cell hybrids and by fluorescence in situ hybridization, Thoreau et al. (1999) mapped the syntaxin-8 gene to chromosome 17p12. ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 604203 was added.

Feb. 25, 2016: Protein entry updated
Automatic update: model status changed

Feb. 24, 2016: Protein entry updated
Automatic update: model status changed

Jan. 25, 2016: Protein entry updated
Automatic update: model status changed