Phospholipid-transporting ATPase IF (ATP11B)

The protein contains 1177 amino acids for an estimated molecular weight of 134190 Da.

 

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), from the outer to the inner leaflet of intracellular membranes (PubMed:30018401). May contribute to the maintenance of membrane lipid asymmetry in endosome compartment (PubMed:30018401). (updated: April 7, 2021)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  4. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 0%
Model score: 0
No model available.

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The reference OMIM entry for this protein is 605869

Atpase, class vi, type 11b; atp11b
Atpir

DESCRIPTION

P-type ATPases, such as ATP11B, are phosphorylated in their intermediate state and drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily transports heavy metal ions, such as Cu(2+) or Cd(2+). Another subfamily transports non-heavy metal ions, such as H(+), Na(+), K(+), or Ca(+). A third subfamily transports amphipaths, such as phosphatidylserine.

CLONING

Nagase et al. (1999) isolated a partial cDNA encoding ATP11B, which they called KIAA0956, from a brain cDNA library. Based on homology analysis, they predicted that the KIAA0956 protein is a chromaffin granule ATPase. RT-PCR analysis detected wide expression, with highest levels in kidney, followed by ovary, corpus callosum, and testis. RUSH proteins are SWI/SNF-related transcription factors with uteroglobin promoter-binding RING finger signatures near their C termini (see 603257). Mansharamani et al. (2001) isolated a nearly full-length rabbit cDNA encoding Rfbp, a RUSH-binding protein that shares 93% amino acid identity with KIAA0956. Nesbit et al. (2004) reported that the ATP11B protein contains 10 transmembrane domains and the conserved DKTGTLT sequence found in P-type ATPases.

GENE STRUCTURE

Nesbit et al. (2004) determined that the ATP11B gene contains at least 30 exons.

MAPPING

Nagase et al. (1999) stated that the ATP11B gene, or KIAA0956, maps to chromosome 3. By genomic sequence analysis, Halleck et al. (1999) confirmed that the ATP11B gene, or ATP1R, maps to chromosome 3. ... More on the omim web site

Subscribe to this protein entry history

April 10, 2021: Protein entry updated
Automatic update: Entry updated from uniprot information.

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 605869 was added.