HBS1-like protein (HBS1L)
The protein contains 684 amino acids for an estimated molecular weight of 75473 Da.
Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway (PubMed:21448132). In the presence of ABCE1 and PELO, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes (PubMed:21448132). Together with PELO and in presence of ABCE1, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway (PubMed:21448132).', 'Facilitates the association of the exosome complex with the SKI complex. (updated: Feb. 10, 2021)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs4435957 |
The reference OMIM entry for this protein is 612450
Hbs1-like protein; hbs1l
Hbs1, s. cerevisiae, homolog of
Erf3-similar protein; erfs
Kiaa1038
CLONING
By searching for genes in a region of chromosome 6 that shows copy number variations associated with pancreatic cancer, Wallrapp et al. (1998) cloned HBS1L, which they called ERFS. The deduced 684-amino acid protein contains 4 GTP-binding motifs and has a calculated molecular mass of about 75 kD. Wallrapp et al. (1998) also identified mouse Hbs1l, which encodes a deduced 600-amino acid protein that has an N-terminal truncation compared with human HBS1L. The mouse and human proteins share 89% identity. Northern blot analysis detected a 3.0-kb transcript in all human tissues examined, with highest expression in heart and skeletal muscle, and lowest expression in lung and kidney. Hbs1l was expressed at all stages of mouse embryonic development examined. By sequencing clones obtained from a size-fractionated fetal brain cDNA library, Kikuno et al. (1999) cloned HBS1L, which they designated KIAA1038. The transcript contains repetitive elements in its 3-prime end, and the deduced protein shares significant similarity with Ef1a (EEF1A1; 130590) from a thermophilic archaeon. RT-PCR ELISA detected low HBS1L expression in adult heart, brain, liver, and spinal cord, and in several specific adult brain regions.MAPPING
By genomic sequence analysis, Wallrapp et al. (1998) mapped the HBS1L gene to chromosome 6q24.MOLECULAR GENETICS
For a discussion of a possible association between variation in the HBS1L gene and fetal hemoglobin levels, see HBFQTL2 (142470). ... More on the omim web site
Feb. 16, 2021: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 612450 was added.