Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2)
The protein contains 271 amino acids for an estimated molecular weight of 28825 Da.
Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure. (updated: Oct. 25, 2017)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs57822546 | |
| dbSNP:rs35030308 | |
| dbSNP:rs34028164 |
The reference OMIM entry for this protein is 610779
Nucleotide-binding protein 2; nubp2
Cytosolic fe-s cluster deficient 1, s. cerevisiae, homolog of; cfd1
DESCRIPTION
NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins (see NUBP1; 600280) (Nakashima et al., 1999).CLONING
Nakashima et al. (1999) cloned mouse Nubp2 and used the murine sequence as a probe in EST database analysis, followed by screening a human teratoma cDNA library to clone full-length human NUBP2. The 271-amino acid human NUBP2 protein shares 72.6% amino acid similarity with mouse Nubp2. Northern blot analysis detected a 1.4-kb NUBP2 transcript with ubiquitous expression in all human adult and fetal tissues tested, with highest expression in adult skeletal muscle. NUBP2 contains conserved an ATP/GTP binding motif A (P-loop), an ATP/GTP binding motif A-prime, and NUBP/MRP alpha and beta motifs. NUBP2 lacks an additional N-terminal sequence with 4 cysteine residues that is present in NUBP1.GENE FUNCTION
Roy et al. (2003) showed that yeast Cfd1 was required for cytosolic Fe-S cluster biogenesis and conversion of mammalian IRP1 (ACO1; 100880) to cytosolic aconitase in yeast. Stehling et al. (2008) found that NBP35 (NUBP1) interacted with coexpressed CFD1 in transfected HeLa cells, suggesting that, like NBP35, CFD1 may function in the regulation of cellular iron homeostasis and cytosolic Fe-S protein assembly.MAPPING
Hartz (2012) mapped the NUBP2 gene to chromosome 16p13.3 based on an alignment of the NUBP2 sequence (GenBank GENBANK AF118394) with the genomic sequence (GRCh37). Nakashima et al. (1999) mapped the mouse Nubp2 gene to the t-complex region of chromosome 17, which shows homology of synteny to human chromosome 16p13.3. ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 610779 was added.