Dynactin subunit 3 (DCTN3)
The protein contains 186 amino acids for an estimated molecular weight of 21119 Da.
Together with dynein may be involved in spindle assembly and cytokinesis. (updated: April 1, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class II
Ciliary basal body-plasma membrane docking
Cytokinesis
Cytoskeleton-dependent cytokinesis
Endoplasmic reticulum to Golgi vesicle-mediated transport
G2/M transition of mitotic cell cycle
Microtubule-based process
Mitotic cell cycle
Mitotic nuclear division
Organelle organization
Regulation of G2/M transition of mitotic cell cycle
The reference OMIM entry for this protein is 607387
Dynactin 3; dctn3
Dynactin complex, 22-kd subunit; dctn22
DESCRIPTION
DCTN3 is a component of the dynactin macromolecular complex. Dynactin, along with dynein (see 600112), is required for diverse cellular functions, including intracellular vesicular transport, spindle formation, chromosome movement, nuclear positioning, and axonogenesis (summary by Karki et al., 1998).CLONING
Using recombinant dynein intermediate chain (see 603331) as ligand, Karki et al. (1998) affinity purified DCTN3, which they called p22, from brain. They used the amino acid sequences of peptide fragments to clone full-length DCTN3 from a neuron cDNA library. The deduced 185-amino acid protein has a calculated molecular mass of about 21 kD and is primarily an alpha-helical protein with no significant coiled-coil regions. Northern blot analysis revealed expression of a 1-kb transcript in all tissues examined, with highest expression in muscle and pancreas and lower levels in brain. Western blot analysis of rat brain cytosol revealed an apparent molecular mass of about 22 kD.GENE FUNCTION
By affinity chromatography of rat brain cytosol, Karki et al. (1998) found that Dctn3 binds directly to the p150 subunit of dynactin (DCTN1; 601143). Immunolocalization of Dctn3 in rat cell lines revealed that it associates with punctate cytoplasmic structures. Dctn3 localized to the centrosome during interphase and to kinetochores and to spindle poles throughout mitosis. Cytoplasmic dynein and dynactin subunits, including DCTN3, localized to the cleavage furrow and to the midbodies of dividing cells.MAPPING
By radiation hybrid analysis, Mills and Jackson (2001) mapped the DCTN3 gene to chromosome 9p13. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 607387 was added.
Feb. 25, 2016: Protein entry updated
Automatic update: model status changed
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed