Glycerol-3-phosphate phosphatase (PGP)
The protein contains 321 amino acids for an estimated molecular weight of 34006 Da.
Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed:26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity). (updated: Oct. 25, 2017)
Protein identification was indicated in the following studies:
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
No binding partner found
The reference OMIM entry for this protein is 172280
Phosphoglycolate phosphatase; pgp
This enzyme (EC 3.1.3.18) may have an important regulatory influence on oxygen transport in man by indirectly affecting the level of red cell 2,3-diphosphoglycerate. (The spellings 'phosphoglycolate' and 'phosphoglycollate' have been used interchangeably, but the former is preferred.) Barker and Hopkinson (1978) devised a method for detecting PGP isozymes after starch-gel electrophoresis. They are present in all human tissues, with highest activities in skeletal and cardiac muscle. Six different electrophoretic phenotypes were identified. Family studies showed that these are determined by 3 alleles at an autosomal locus. In a sample of Europeans, the frequency of the alleles were PGP(1), 0.826; PGP(2), 0.129; PGP(3), 0.045. The 3-banded isozyme pattern in heterozygotes suggested that the enzyme is dimeric. The phosphoglycolate phosphatase locus was assigned to chromosome 16 by Donald et al. (1979) and by Blankenstein-Wijnen et al. (1979). Family data suggested that PGP is not close to 16qh or alpha-haptoglobin (Povey et al., 1980). The most likely regional assignment was considered to be 16p13 or 16p12, but a site on 16q could not be excluded (Povey et al., 1980). Koeffler et al. (1981) assigned the PGP locus to 16p by mouse-man somatic cell hybridization. Bale et al. (1984) suggested the existence of recombination heterogeneity in the linkage with haptoglobin. Reeders et al. (1986) showed that the PGP locus is, like the alpha-globin locus, close to the adult polycystic kidney disease locus (maximum lod = 8.21, theta = 0.00). PGP versus the 3-prime-HVR (hypervariable region 3-prime to the alpha-globin locus) gave a maximum lod score of 11.61 at theta = 0.00. Thus, PGP, HBA, and APCKD are all close together on 16p. Mulley et al. (1990) assigned the PGP gene to 16p13.3 by electrophoretic detection of enzymes from a human/mouse somatic cell panel, the members of which carried portions of human chromosome 16 with precisely defined breakpoints. Eiberg et al. (1993) reported a suggestion of linkage between manic-depressive illness (125480) and PGP; a maximum lod score of 2.20 at 0% recombination was found in a single large family. A second family was uninformative. Data on gene frequencies of allelic variants were tabulated by Roychoudhury and Nei (1988). ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 172280 was added.