F-box only protein 50 (NCCRP1)
The protein contains 275 amino acids for an estimated molecular weight of 30847 Da.
Promotes cell proliferation. (updated: Jan. 7, 2015)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
No binding partner found
Biological Process
Glycoprotein catabolic process
Positive regulation of cell population proliferation
Protein ubiquitination
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
Ubiquitin-dependent ERAD pathway
Molecular Function
The reference OMIM entry for this protein is 615901
Nonspecific cytotoxic cell receptor protein 1, zebrafish, homolog of; nccrp1
F-box only protein 50; fbxo50
DESCRIPTION
NCCRP1 is predicted to function as a scaffold protein for ubiquitin ligase complexes (Kallio et al., 2011).CLONING
By searching databases for sequences similar to mouse Nccrp1, Kallio et al. (2011) identified orthologs in several vertebrates, including human. The deduced 275-amino acid human protein has an N-terminal proline-rich domain followed by an F-box domain. NCCRP1 shares highest similarity with lectin-like F-box proteins (e.g., FBXO2; 607112), which function as scaffold proteins in ubiquitin ligase complexes. Mass spectrometric analysis indicated the presence of an intramolecular disulfide bond between cys158 and cys192. In silico analysis of normal human tissues revealed relatively narrow NCCRP1 expression in tissues containing squamous epithelium, including esophagus, oral cavity, skin, tongue, and male and female reproductive organs. Quantitative RT-PCR detected more widespread Nccrp1 expression in mouse, with highest expression in kidney, followed by stomach, colon, duodenum, and prostate. Immunohistochemical analysis of HeLa cells showed that endogenous NCCRP1 localized to cytoplasm. Following its expression in E. coli, NCCRP1 had an apparent molecular mass of approximately 30 kD by SDS-PAGE. Kallio et al. (2011) found no evidence for expression of mammalian NCCRP1 on immune cell membranes, as had been reported in fish.GENE FUNCTION
Kallio et al. (2011) found that silencing of NCCRP1 significantly reduced proliferation in HeLa cells.MAPPING
Kallio et al. (2011) reported that NCCRP1 maps to chromosome 19q13.2. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for NCCRP1
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 615901 was added.