Membrane-associated progesterone receptor component 1 (PGRMC1)

The protein contains 195 amino acids for an estimated molecular weight of 21671 Da.

 

Component of a progesterone-binding protein complex (PubMed:28396637). Binds progesterone (PubMed:25675345). Has many reported cellular functions (heme homeostasis, interaction with CYPs). Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan (By similarity). Intracellular heme chaperone. Regulates heme synthesis via interactions with FECH and acts as a heme donor for at least some hemoproteins (PubMed:27599036). (updated: April 22, 2020)

Protein identification was indicated in the following studies:

  1. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  2. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  3. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  4. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 0%
Model score: 40

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The reference OMIM entry for this protein is 300435

Progesterone receptor membrane component 1; pgrmc1

CLONING

Using the sequence of a porcine progesterone receptor as probe, Gerdes et al. (1998) cloned PGRMC1, which they designated HPR6.6. The deduced protein contains 195 amino acids and has an N-terminal 26-residue hydrophobic region. It has no N-glycosylation site. PGRMC1 shares about 50% identity overall with PGRMC2 (607735), and they share 68% identity in their C termini, including a highly conserved 58-amino acid sequence. Northern blot analysis revealed expression of 2.0- and 5.0-kb transcripts in all tissues examined, with highest expression in liver and kidney. Transfection in HEK293 cells resulted in a protein with an apparent molecular mass of about 28 kD.

MAPPING

By PCR analysis of human-hamster hybrid panels and by genomic sequence analysis, Gerdes et al. (1998) mapped the PGRMC1 gene to chromosome Xq22-q24. ... More on the omim web site

Subscribe to this protein entry history

June 30, 2020: Protein entry updated
Automatic update: OMIM entry 300435 was added.

April 25, 2020: Protein entry updated
Automatic update: Entry updated from uniprot information.

Dec. 10, 2018: Protein entry updated
Automatic update: model status changed

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).