Transmembrane reductase CYB561D2 (CYB561D2)
The protein contains 222 amino acids for an estimated molecular weight of 23974 Da.
Transmembrane reductase that may use ascorbate as an electron donor in the cytoplasm and transfer electrons across endoplasmic reticulum membranes to reduce monodehydro-L-ascorbate radical and iron cations Fe(3+) in the lumen of that compartment. (updated: June 2, 2021)
Protein identification was indicated in the following studies:
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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No binding partner found
The reference OMIM entry for this protein is 607068
Cytochrome b561 domain-containing protein 2; cyb561d2
101f6
CLONING
By physical cloning methodologies and bioinformatic computational analyses, Lerman and Minna (2000) identified a number of genes, including CYB561D2, which they called 101F6, in a region of chromosome 3p21.3 that is associated with a putative lung cancer tumor suppressor gene. The deduced 222-amino acid integral protein is 95% identical to the mouse protein and contains 6 transmembrane helices with both N and C termini in the cytoplasm. Northern blot analysis revealed wide expression of a 1.5-kb transcript that was strongest in liver, followed by placenta and lung, with low amounts in heart, kidney, and pancreas and only traces in skeletal muscle and brain. CYB561D2 was highly expressed in most lung cancer cell lines tested. No mutations were detected in any of the lung cancer cell lines. Lerman and Minna (2000) concluded that CYB561D2 is an unlikely candidate tumor suppressor gene.GENE STRUCTURE
By genomic sequence analysis, Lerman and Minna (2000) determined that the CYB561D2 gene contains 4 exons and spans 3.2 kb.MAPPING
By genomic sequence analysis, Lerman and Minna (2000) determined that the CYB561D2 gene resides in a 120-kb critical region for a lung cancer tumor suppressor gene on chromosome 3p21.3. ... More on the omim web site
July 1, 2021: Protein entry updated
Automatic update: Entry updated from uniprot information.
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 607068 was added.
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Dec. 9, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).