Erlin-1 (ERLIN1)

The protein contains 346 amino acids for an estimated molecular weight of 38926 Da.

 

Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. Binds cholesterol and may promote ER retention of the SCAP-SREBF complex (PubMed:24217618).', '(Microbial infection) Required early in hepatitis C virus (HCV) infection to initiate RNA replication, and later in the infection to support infectious virus production. (updated: Oct. 7, 2020)

Protein identification was indicated in the following studies:

  1. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  2. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  3. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 0%
Model score: 43

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VariantDescription
SPG62

The reference OMIM entry for this protein is 611604

Endoplasmic reticulum lipid raft-associated protein 1; erlin1
Keo4

CLONING

Using PCR-based subtractive hybridization to isolate genes differentially expressed by antigen-stimulated human dendritic cells, Li et al. (2000) cloned ERLIN1, which they called KEO4. The deduced 346-amino acid protein has a calculated molecular mass of 38.9 kD. It has an N-terminal mitochondrial targeting sequence, followed by a transmembrane region and sites for phosphorylation, N-myristoylation, N-glycosylation, and glycosaminoglycan attachment. Northern blot analysis detected a 3.8-kb transcript in heart, placenta, liver, kidney, pancreas, prostate, testis, ovary, small intestine, and colon. RT-PCR showed variable expression in all cell lines examined. Western blot analysis revealed a 39-kD protein in antigen-activated dendritic cells, but not in unactivated dendritic cells. Using monoclonal antibodies to human lipid raft proteins, Browman et al. (2006) identified ERLIN1 and ERLIN2 (611605) as components of lipid rafts. Immunohistochemical analysis of endogenous and fluorescence-tagged proteins revealed that ERLIN1 and ERLIN2 localized specifically to the endoplasmic reticulum and nuclear envelope. The 2 proteins share 83% identity, and both contain a conserved prohibitin (PHB; 176705) homology domain of about 160 amino acids.

MAPPING

The International Radiation Hybrid Mapping Consortium mapped the ERLIN1 gene to chromosome 10 (TMAP WI-16766). ... More on the omim web site

Subscribe to this protein entry history

Oct. 20, 2020: Protein entry updated
Automatic update: Entry updated from uniprot information.

Feb. 23, 2019: Protein entry updated
Automatic update: model status changed

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).

Oct. 19, 2018: Protein entry updated
Automatic update: OMIM entry 611604 was added.