Protein YIF1A (YIF1A)

The protein contains 293 amino acids for an estimated molecular weight of 32011 Da.

 

Possible role in transport between endoplasmic reticulum and Golgi. (updated: Sept. 12, 2018)

Protein identification was indicated in the following studies:

  1. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  2. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

This protein is predicted to be membranous by TOPCONS.

Topcons

Interpro domains
Total structural coverage: 0%
Model score: 43
MODL_ROSETTA-3.4

(right-click above to access to more options from the contextual menu)

No binding partner found

The reference OMIM entry for this protein is 611484

Yip1-interacting factor, s. cerevisiae, homolog of, a; yif1a
Yif1

CLONING

Matern et al. (2000) cloned yeast Yif1, and by database analysis, they identified the human ortholog YIF1A, which encodes a deduced 293-amino acid protein with 5 transmembrane domains in its C-terminal half. By database analysis with yeast Yif1 as query, followed by PCR of a human testis cDNA library, Jin et al. (2005) cloned YIF1A. Database analysis showed that YIF1A is conserved across species. Fluorescence-tagged YIF1A was expressed with Golgi marker proteins in transfected HeLa cells, and its localization became diffuse during the dissolution of the Golgi apparatus in metaphase.

GENE FUNCTION

Matern et al. (2000) showed that yeast Yif1 has a vital function in endoplasmic reticulum (ER)-to-Golgi protein trafficking and that, via its hydrophobic C-terminal half, Yif1 forms a tight complex with Yip1 (YIPF5; 611483) on Golgi membranes. The N-terminal hydrophilic half of Yif1 faces the cytosol, and loss of Yif1 impaired transport vesicle docking to the acceptor membrane. Using reciprocal yeast 2-hybrid analysis and coimmunoprecipitation assays, Jin et al. (2005) showed that human YIPF5 and YIF1A interact directly. Both proteins localized to the Golgi apparatus in transfected human embryonic kidney cells, and the Golgi localization of YIF1A depended upon the transmembrane domain of YIPF5. Mutation analysis revealed that both the N and C termini of YIF1A were required to anchor YIF1A to Golgi.

MAPPING

The International Radiation Hybrid Mapping Consortium mapped the YIF1A gene to chromosome 11 (TMAP SHGC-64947). ... More on the omim web site

Subscribe to this protein entry history

Dec. 10, 2018: Protein entry updated
Automatic update: model status changed

Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 611484 was added.

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).