Kinesin-like protein KIF20A (KIF20A)

The protein contains 890 amino acids for an estimated molecular weight of 100278 Da.

 

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility. (updated: Sept. 12, 2018)

Protein identification was indicated in the following studies:

  1. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

Interpro domains
Total structural coverage: 0%
Model score: 36

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VariantDescription
dbSNP:rs3734116
dbSNP:rs3172747

No binding partner found

The reference OMIM entry for this protein is 605664

Kinesin family member 20a; kif20a
Rab6-interacting protein, kinesin-like; rab6kifl
Rabkinesin 6

DESCRIPTION

Kinesin-like proteins, such as KIF20A, are microtubule-associated motors that play important roles in intracellular transport and cell division (summary by Lai et al., 2000).

CLONING

Lai et al. (2000) identified a novel kinesin-like protein, RAB6KIFL, within a 1.5-Mb region of 5q31 that is frequently deleted in myeloid leukemias. The composite cDNA encodes a deduced 890-amino acid protein that contains a motor domain and an ATP catalytic domain, hallmarks of kinesin-like proteins, in the N terminus. The protein shares 87% sequence identity with the mouse Rab6kifl protein. In contrast to the ubiquitous expression of the mouse protein, expression of the human, approximately 3.4-kb RAB6KIFL transcript appears to be tissue specific. Northern blot analysis detected high expression in fetal tissues and in adult thymus, bone marrow, and testis, and low expression in heart, placenta, and spleen. No expression was detected in brain, lung, liver, skeletal muscle, kidney, pancreas, or peripheral leukocytes. Lai et al. (2000) detected no mutations of the RAB6KIFL gene in malignant myeloid cells with loss of 5q, suggesting that the gene is not a myeloid tumor suppressor.

GENE STRUCTURE

Lai et al. (2000) determined that the RAB6KIFL gene spans approximately 8.5 kb of genomic DNA and contains 19 exons.

GENE FUNCTION

Using differential display, Horrevoets et al. (1999) found that expression of KIF20A in cultured human umbilical vein endothelial cells was repressed by cytokines and TNF-alpha (191160), a proapoptotic protein. Nguyen et al. (2014) developed a cell-free system to recapitulate cytokinesis signaling using cytoplasmic extract from Xenopus eggs. Microtubules grew out as asters from artificial centrosomes and met to organize antiparallel overlap zones. These zones blocked the interpenetration of neighboring asters and recruited cytokinesis midzone proteins, including the chromosomal passenger complex and centralspindlin. The chromosomal passenger complex was transported to overlap zones, which required 2 motor proteins, KIF4A (300521) and a KIF20A paralog. Using supported lipid bilayers to mimic the plasma membrane, Nguyen et al. (2014) observed the recruitment of cleavage furrow markers, including an active RhoA reporter, at microtubule overlaps.

MAPPING

The KIF20A gene maps to chromosome 5q31 (Lai et al., 2000). ... More on the omim web site

Subscribe to this protein entry history

June 30, 2020: Protein entry updated
Automatic update: OMIM entry 605664 was added.

Feb. 23, 2019: Protein entry updated
Automatic update: model status changed

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).