Arf-GAP domain and FG repeat-containing protein 2 (AGFG2)
The protein contains 481 amino acids for an estimated molecular weight of 48963 Da.
No function (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs34731997 |
The reference OMIM entry for this protein is 604019
Adp-ribosylation factor gtpase-activating protein with fg repeats 2; agfg2
Arfgap with fg repeats 2
Hiv-1 rev-binding protein-like; hrbl
Rev/rex activation domain-binding protein-related; rabr
CLONING
Salcini et al. (1997) determined that the EH protein-protein interaction domains from EPS15 (600051) and mouse Eps15R bind in vitro to peptides containing an asn-pro-phe (NPF) motif. By screening a human fibroblast expression library with a chimeric glutathione S-transferase-EH-domain protein, the authors identified cDNAs encoding NUMB (603728), NUMBR (604018), RAB (600862), RABR, and 3 other proteins that interact with EH domains. The predicted 481-amino acid RABR protein shares 46% identity with RAB. Both RAB and RABR contain a zinc finger region near the N terminus, several phe-gly (FG) motifs characteristic of nucleoporin-like proteins, and 4 NPF motifs located in the C-terminal half.MAPPING
Gross (2014) mapped the AGFG2 gene to chromosome 7q22.1 based on an alignment of the AGFG2 sequence (GenBank GENBANK BC009393) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 604019 was added.