Heterogeneous nuclear ribonucleoprotein F (HNRNPF)
The protein contains 415 amino acids for an estimated molecular weight of 45672 Da.
Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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| Variant | Description |
|---|---|
| dbSNP:rs17851426 |
Biological Process
Fibroblast growth factor receptor signaling pathway
Interleukin-12-mediated signaling pathway
MRNA splicing, via spliceosome
Regulation of RNA splicing
RNA metabolic process
RNA processing
The reference OMIM entry for this protein is 601037
Heterogeneous nuclear ribonucleoprotein f; hnrnpf
Hnrpf
CLONING
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that bind heterogeneous nuclear RNA (hnRNA), the transcripts produced by RNA polymerase II (see 180660). More than 20 such proteins have been described and designated with letters from A to U. By molecular cDNA cloning, 2-dimensional gel immunoblotting, and amino acid microsequencing, Honore et al. (1995) identified 3 sequence-unique and distinct proteins that constitute a subfamily of ubiquitously expressed hnRNPs. The identity between hnRNPs H (601035) and H-prime (300610) was 96%, between H and F, 78%, and between H-prime and F, 75%.MAPPING
By fluorescence in situ hybridization, Honore et al. (1995) mapped the HNRNPF gene to 10q11.21-q11.22. McDonald et al. (1992) had previously mapped the HNRNPF gene to 10q11.2. ... More on the omim web site
Nov. 17, 2018: Protein entry updated
Automatic update: OMIM entry 601037 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).