40S ribosomal protein S8 (RPS8)
The protein contains 208 amino acids for an estimated molecular weight of 24205 Da.
No function (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs11537870 |
Biological Process
Cytoplasmic translation
Maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
SRP-dependent cotranslational protein targeting to membrane
Translation
Translational initiation
Viral transcription
The reference OMIM entry for this protein is 600357
Ribosomal protein s8; rps8
CLONING
The eukaryotic ribosome is composed of 4 RNA molecules (see 180450) and approximately 80 proteins. Davies and Fried (1993) used a PCR-based cloning strategy to isolate the intron-containing gene encoding human ribosomal protein S8 in the presence of its many processed pseudogenes and determined the DNA sequence (GenBank GENBANK X67247) of the entire gene, as well as of its upstream and downstream flanking regions. The RPS8 gene is 3,161 bp long. By Northern blot analysis using a rat Rps8 probe, Pogue-Geile et al. (1991) found increased levels of RPS8 mRNA in all 3 human colorectal tumors and all 3 human colon polyps examined, as compared to matched normal colonic mucosa. RPS8 is expressed as an approximately 980-bp transcript.GENE STRUCTURE
Davies and Fried (1993) determined that the RPS8 gene compromises 6 exons. Despite lacking a consensus TATA box, primer extension analysis indicated that the start of transcription is precisely located at a C residue within an 11-bp oligopyrimidine tract. The first exon, which contains the ATG start codon, is only 27 bp long.MAPPING
Davies and Fried (1993) localized the human RPS8 gene to 1p34.1-p32 using a combination of Southern blotting, PCR, and fluorescence in situ hybridization. Kenmochi et al. (1998) confirmed the mapping assignment reported by Davies and Fried (1993). ... More on the omim web site
June 29, 2020: Protein entry updated
Automatic update: OMIM entry 600357 was added.
Feb. 22, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).