Serine incorporator 3 (SERINC3)
The protein contains 473 amino acids for an estimated molecular weight of 52580 Da.
Restriction factor required to restrict infectivity of lentiviruses, such as HIV-1: acts by inhibiting an early step of viral infection. Impairs the penetration of the viral particle into the cytoplasm (PubMed:26416733, PubMed:26416734). (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt, is predicted to be membranous by TOPCONS.
(right-click above to access to more options from the contextual menu)
| Variant | Description |
|---|---|
| empty |
Biological Process
Defense response to virus
Detection of virus
Innate immune response
L-serine biosynthetic process
Phosphatidylserine metabolic process
Positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
Sphingolipid metabolic process
Viral process
The reference OMIM entry for this protein is 607165
Serine incorporator 3; serinc3
Tumor differentially expressed 1; tde1
Tms1
DESCRIPTION
SERINC3 belongs to the SERINC family of transmembrane proteins that facilitate incorporation of serine into phosphatidylserine and sphingolipids (Inuzuka et al., 2005).CLONING
By screening a human placenta cDNA library with the mouse Tde cDNA sequence, Bossolasco et al. (1999) cloned SERINC3, which they called TDE1. The 473-amino acid protein has a predicted molecular mass of 52 kD. Use of an alternative polyadenylation site results in a splice variant lacking 61 amino acids. Like the mouse protein, with which it shares 78% sequence homology, human TDE1 contains several predicted hydrophobic alpha helices characteristic of transmembrane proteins, and it has several potential phosphorylation sites. Northern blot analysis detected ubiquitous expression of TDE1 in all tissues and cell lines examined, with overexpression in 3 of 5 lung tumors. Grossman et al. (2000) cloned mouse Serinc3, which they called Tms1, and they identified human TMS1 by database analysis. The deduced proteins contain 11 transmembrane segments and a MYC (190080)-type helix-loop-helix dimerization motif between transmembrane domains 7 and 8. Epitope-tagged Tms1 was expressed in a punctate distribution on the surface of transfected HEK293 cells. Bossolasco et al. (2006) showed that the TDE1 protein contains a putative signal peptide followed by 11 transmembrane domains, and it has 12 putative phosphorylation sites. The N terminus is extracellular, and the C terminus is cytoplasmic. Fluorescence-tagged TDE1 localized predominantly at the plasma membrane, although some was apparent at the perinuclear area.GENE FUNCTION
Bossolasco et al. (2006) found that TDE1 overexpression in transfected rat cells resulted in reduced contact inhibition with formation of robust foci. In transfected rat and human cell lines, TDE1 overexpression reduced apoptosis caused by serum starvation or etoposide treatment, as shown by reduced caspase-3 (600636) activation and DNA laddering. TDE1 did not alter cell growth rate, immortalization, or motility.MAPPING
By FISH, Bossolasco et al. (1999) mapped the SERINC3 gene to chromosome 20q13.1-q13.3. ... More on the omim web site
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 607165 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).