6-phosphogluconolactonase (PGLS)
The protein contains 258 amino acids for an estimated molecular weight of 27547 Da.
Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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The reference OMIM entry for this protein is 604951
6-@phosphogluconolactonase; pgls
6pgl
CLONING
6-Phosphogluconolactonase (EC 3.1.1.31) catalyzes the hydrolysis of 6-phosphogluconolactone, which is the second step of the pentose phosphate pathway. The bacterial devB gene, which is often found in proximity to the glucose-6-phosphate dehydrogenase gene (G6PD), encodes a protein that is homologous to the C-terminal part of human hexose-6-phosphate dehydrogenase (H6PD; 138090). By searching a human EST database for cDNAs encoding homologs of the devB protein from A. actinomycetemcomitans, Collard et al. (1999) identified PGLS cDNAs. They isolated a human liver cDNA containing a complete PGLS coding sequence. The deduced 258-amino acid PGLS protein has a calculated molecular mass of 27,529 Da. PGLS shares 33 to 37% amino acid sequence identity with yeast Sol1 to Sol4, 26% identity with the C-terminal part of human H6PD, 20 to 25% identity with bacterial devB proteins, and 17% identity with human glucosamine-6-phosphate isomerase.MAPPING
By radiation hybrid mapping, Collard et al. (1999) mapped the PGLS gene to 19p13.2. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 15, 2016: Protein entry updated
Automatic update: OMIM entry 604951 was added.