Transmembrane protein 205 (TMEM205)

The protein contains 189 amino acids for an estimated molecular weight of 21198 Da.

 

In cancer cells, plays a role in resistance to the chemotherapeutic agent cisplatin. (updated: Oct. 10, 2018)

Protein identification was indicated in the following studies:

  1. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  2. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  3. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  4. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete
TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.

This protein is predicted to be membranous by TOPCONS.


Interpro domains
Total structural coverage: 0%
Model score: 44

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No binding partner found

Biological Process

Cellular Component

Integral component of membrane GO Logo

Molecular Function

The reference OMIM entry for this protein is 613771

Transmembrane protein 205; tmem205

CLONING

By screening for genes upregulated in cisplatin-resistant human cancer cell lines compared with parental cell lines, Shen et al. (2010) cloned TMEM205. The deduced 189-amino acid protein has 4 transmembrane domains. Quantitative real-time PCR detected variable TMEM205 expression in all 29 human tissues examined, with highest expression in pancreas, followed by adrenal gland, thyroid, liver, mammary gland, prostate, kidney, and retina. Lowest expression was detected in skeletal muscle. Western blot analysis detected TMEM205 at an apparent molecular mass of about 21 kD in membrane fractions of early cisplatin-resistant cells. Immunoelectron microscopy showed that TMEM205 localized to cell surface microvilli.

GENE FUNCTION

Using RT-PCR and immunoblot analyses, Shen et al. (2010) confirmed that expression of TMEM205 was upregulated in cisplatin-resistant human and mouse cells compared with parental cell lines. Transfection of TMEM205 into naive cells induced cisplatin resistance. Development of cisplatin resistance was associated with reduced intracellular cisplatin levels and redistribution of TMEM205 from the cell surface to the trans-Golgi region. Shen et al. (2010) hypothesized that TMEM205 may be a cisplatin transporter that permits drug resistance by reducing the intracellular cisplatin concentration.

MAPPING

Hartz (2011) mapped the TMEM205 gene to chromosome 19p13.2 based on an alignment of the TMEM205 sequence (GenBank GENBANK AY358949) with the genomic sequence (GRCh37). ... More on the omim web site

Subscribe to this protein entry history

June 30, 2020: Protein entry updated
Automatic update: OMIM entry 613771 was added.

Dec. 10, 2018: Protein entry updated
Automatic update: model status changed

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).